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首页> 外文期刊>Electroanalysis >Preparation of a selective receptor for ephedrine for the rapid electrochemical detection of ephedrine in human urine using stabilized in air lipid films with incorporated ephedrine receptor
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Preparation of a selective receptor for ephedrine for the rapid electrochemical detection of ephedrine in human urine using stabilized in air lipid films with incorporated ephedrine receptor

机译：掺入麻黄碱受体的稳定化空气脂质膜制备麻黄碱选择性受体，用于快速电化学检测人尿中的麻黄碱

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摘要

The present technique describes the preparation of a selective receptor for ephedrine and a technique for the rapid electrochemical detection of ephedrine in human urine using lipid films with incorporated receptor that are synthesized by a chemical reaction with a methacrylate polymer on a glass fiber filter. The selective receptor was synthesized using a resorcin[4]arene receptor and by transforming all the -OH groups into methoxy groups. Injections of ephedrine were made into flowing streams of a carrier electrolyte solution and a transient current signal, with duration of seconds, appeared in less than two minutes after exposure of the lipid membranes to the compound. The magnitude of this signal was linearly related to the concentration of this stimulant, which could be determined at micromolar levels. The effect of potent interferences including a wide range of compounds usually found in human urine (i.e., ascorbic aid, glucose, leucine, glycine, tartrate, citrate, bicarbonate and caffeine) was examined. Also, the effect of proteins and lipids was investigated. The results showed that there were no interferences from all these constituents in concentration levels Usually found in human urine samples. Dopamine was also investigated as a potent interferent. The results have shown that the transformation of the hydroxy to methoxy groups has increased the selectivity of the receptor towards ephedrine and decreased it towards dopamine which does not cause and interference at concentration levels lower than of 10(-3) M. Urine samples provided similar results and allowed the development of a technique for the rapid electrochemical detection of this stimulant in human urine at the levels of 10(-6) M concentrations. The reproducibility of the method was checked in about 100 samples.
机译：本技术描述了用于麻黄碱的选择性受体的制备和一种用于人尿中的麻黄碱的快速电化学检测的技术，该技术使用具有掺入的受体的脂质膜，该膜通过与甲基丙烯酸酯聚合物在玻璃纤维过滤器上的化学反应合成。使用间苯二酚[4]芳烃受体并通过将所有-OH基团转化为甲氧基基团来合成选择性受体。将麻黄碱注射液注入载体电解质溶液的流动物流中，在脂质膜暴露于该化合物后不到两分钟的时间内，出现了持续时间为几秒钟的瞬态电流信号。该信号的强度与该兴奋剂的浓度线性相关，该浓度可以在微摩尔水平确定。检查了强干扰物的影响，其中包括通常在人尿液中发现的多种化合物（即抗坏血酸，葡萄糖，亮氨酸，甘氨酸，酒石酸盐，柠檬酸盐，碳酸氢盐和咖啡因）。此外，还研究了蛋白质和脂质的作用。结果表明，所有这些成分的浓度水平在人尿液样品中通常没有干扰。多巴胺也被作为有效的干扰素进行了研究。结果表明，羟基向甲氧基的转化增加了受体对麻黄碱的选择性，并降低了其对多巴胺的选择性，在浓度低于10（-3）M时不会引起和干扰。尿液样品提供了类似的结果结果并允许开发一种技术，用于在人体尿液中以10（-6）M的浓度快速电化学检测这种兴奋剂。在大约100个样品中检查了该方法的可重复性。

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