• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electroanalysis >Electrochemistry and electrocatalysis of a nafionano-CaCO3/Hb film modified carbon ionic liquid electrode using BMIMPF6 as binder
【24h】

Electrochemistry and electrocatalysis of a nafionano-CaCO3/Hb film modified carbon ionic liquid electrode using BMIMPF6 as binder

机译：以BMIMPF6为粘合剂的nafion / nano-CaCO3 / Hb膜修饰碳离子液体电极的电化学和电催化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper a room temperature ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6) was used as binder for the construction of carbon ionic liquid electrode (CILE) and a new electrochemical biosensor was developed for determination of H2O2 by immobilization of hemoglobin (Hb) in the composite film of Nafionano-CaCO3 on the surface of CILE. The Hb modified electrode showed a pair of well-defined, quasi-reversible redox peaks with E-pa and E-pc as -0.265 V and -0.470 V (vs. SCE). The formal potential (E degrees ') was got by the midpoint of E-pa and E-pc as -0.368 V, which was the characteristic of Hb Fe(III)/Fe(II) redox couples. The peak to peak separation was 205 mV in pH 7.0 Britton - Robinson (B-R) buffer solution at the scan rate of 100 mV/s. The direct electrochemistry of Hb in the film was carefully investigated and the electrochemical parameters of Hb on the modified electrode were calculated as alpha = 0.487 and k(s) = 0.128s(-1). The Nafionano-CaCO3/Hb film electrode showed good electrocatalysis to the reduction of H2O2 in the linear range from 8.0 to 240.0 mu mol/L and the detection limit as 5.0 mu mol/L (3 sigma). The apparent Michaelis - Menten constant (K-M(app)) was estimated to be 65.7 mu mol/L. UV-vis absorption spectroscopy and FT-IR spectroscopy showed that Hb in the Nafionano-CaCO3 composite film could retain its native structure.
机译：本文将室温离子液体1-丁基-3-甲基咪唑六氟磷酸盐（BMIMPF6）用作构建碳离子液体电极（CILE）的粘合剂，并开发了一种新的电化学生物传感器，用于固定血红蛋白来测定H2O2（ Hb）在CILE表面的Nafion / nano-CaCO3复合膜中。血红蛋白修饰的电极显示出一对明确定义的准可逆氧化还原峰，其E-pa和E-pc为-0.265 V和-0.470 V（相对于SCE）。通过E-pa和E-pc的中点获得的形式势（E度'）为-0.368 V，这是Hb Fe（III）/ Fe（II）氧化还原对的特征。在pH 7.0 Britton-Robinson（B-R）缓冲溶液中，峰对峰的分离为205 mV，扫描速率为100 mV / s。仔细研究了薄膜中Hb的直接电化学，并计算了修饰电极上Hb的电化学参数，α= 0.487，k（s）= 0.128s（-1）。 Nafion / nano-CaCO3 / Hb薄膜电极对H2O2的还原表现出良好的电催化作用，线性范围为8.0至240.0μmol/ L，检出限为5.0μmol/ L（3σ）。表观Michaelis-Menten常数（K-M（app））估计为65.7μmol/ L。紫外可见吸收光谱和傅立叶变换红外光谱表明，Nafion /纳米CaCO3复合膜中的Hb可以保留其天然结构。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号