Acetylcholinesterase immobilization on 3-mercaptopropionic acid self assembled monolayer for determination of pesticides

Pedrosa VA; Caetano J; Machado SAS; Freire RS; Bertotti M

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Acetylcholinesterase immobilization on 3-mercaptopropionic acid self assembled monolayer for determination of pesticides

机译：乙酰胆碱酯酶固定在3-巯基丙酸自组装单层膜上测定农药

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Studies on the immobilization of acetylcholinesterase onto a SAM gold electrode and the use of the fabricated biosensor for the determination of carbaryl and parathion are presented. The influence of pH, ionic strength, enzyme loading and concentration of glutaraldehyde on the response of the biosensor was investigated. The amperometric biosensor developed in this study provided linearity to parathion and carbaryl in the 2.0 a 30.0 x 10(-6) mol L-1 concentration range. The detection limits under the optimum working conditions were found to be 9.3 mu g L-1 for parathion and 9.0 mu g L-1 for carbaryl. The enzyme electrode was found to be stable for 7 days.

机译：提出了将乙酰胆碱酯酶固定在SAM金电极上的研究以及所制造的生物传感器用于测定甲萘威和对硫磷的研究。研究了pH，离子强度，酶负载和戊二醛浓度对生物传感器响应的影响。在这项研究中开发的安培生物传感器在2.0 a 30.0 x 10（-6）mol L-1浓度范围内为对硫磷和西芳基化合物提供线性。发现在最佳工作条件下，对硫磷的检出限为9.3μg L-1，西芳基的检出限为9.0μg L-1。发现酶电极稳定7天。

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《Electroanalysis》 |2007年第13期|共6页
作者
Pedrosa VA; Caetano J; Machado SAS; Freire RS; Bertotti M;
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正文语种 eng
中图分类电化学、电解、磁化学;
关键词
biosensor; pesticides; self-assembled monolayer; enzyme; acetylcholinesterase; ORGANOPHOSPHATE PESTICIDES; ELECTROCHEMICAL BIOSENSOR; MODIFIED ELECTRODES; BIENZYME BIOSENSOR; DIAMOND ELECTRODE; INSECTICIDES; LACCASE;

机译：生物传感器;农药;自组装单层;酶;乙酰胆碱酯酶;有机磷农药;电化学生物传感器;修饰的电极;双酶生物传感器;金刚石电极;农药;酶;

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1. Acetylcholinesterase immobilization on 3-mercaptopropionic acid self assembled monolayer for determination of pesticides [J] . Pedrosa VA, Caetano J, Machado SAS, Electroanalysis . 2007,第13期

机译：乙酰胆碱酯酶固定在3-巯基丙酸自组装单层膜上测定农药
2. Voltammetric determination of epinephrine with a 3-mercaptopropionic acid self-assembled monolayer modified gold electrode [J] . Zeng BZ., Yang YX., Zhao FQ. Electroanalysis . 2003,第12期

机译：3-巯基丙酸自组装单层修饰金电极伏安法测定肾上腺素
3. Determination of real composition of 3-mercaptopropionic acid and 1-octadecanethiol mixed self-assembled monolayers by using electrochemical and electrochemical quartz crystal microbalance measurements [J] . Sato Y., Mizutani F. Electroanalysis . 1998,第9期

机译：电化学和电化学石英晶体微量天平测量法测定3-巯基丙酸和1-十八烷硫醇混合自组装单层的真实组成
4. The Construction of Layered Architectures of Dendrimers-Adsorption Layers of Amino-terminated Dendrimers on 3-Mercaptopropionic Acid Self-assembled Monolayer Formed on Au [C] . Hiroki Nagaoka, Toyoko Imae International Conference on Micro amp; Nano Systems 2002 (ICMNS 2002) Aug 11-14, 2002 Kunming, China . 2002

机译：在Au上形成的3-巯基丙酸自组装单分子膜上的氨基封端的树枝状大分子的树枝状大分子-吸附层的分层体系结构的构建
5. Self-Assembled Monolayer-Based Surface Modification of Stainless Steel for Oriented Antibody Immobilization: Towards Improved Stent Biocompatibility. [D] . Benvenuto, Pasquale. 2015

机译：自组装基于单层的不锈钢表面修饰的定向抗体固定化：改善支架生物相容性。
6. Determination of Parathion and Carbaryl Pesticides in Water and Food Samples Using a Self Assembled Monolayer/Acetylcholinesterase Electrochemical Biosensor [O] . Valber A. Pedrosa, Josiane Caetano, Sergio A. S. Machado, 2008

机译：使用自组装单层/乙酰胆碱酯酶电化学生物传感器测定水和食品样品中的对硫磷和甲萘威农药
7. Determination of parathion and carbaryl pesticides in water and food samples using a self assembled monolayer/acetylcholinesterase electrochemical biosensor [O] . PEDROSA, Valber A., CAETANO, Josiane, MACHADO, Sergio A. S., 2008

机译：使用自组装单层/乙酰胆碱酯酶电化学生物传感器测定水和食品样品中的对硫磷和西维因农药

Acetylcholinesterase immobilization on 3-mercaptopropionic acid self assembled monolayer for determination of pesticides

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