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Direct Bioelectrocatalysis of PQQ-Dependent Glucose Dehydrogenase

机译：PQQ依赖性葡萄糖脱氢酶的直接生物电催化

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摘要

The direct bioelectrocatalysis was demonstrated for pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-dependent GDH) covalently attached to single-walled carbon nanotubes (SWNTs). The homogeneous ink-like SWNT suspension was used for both creating the SWNT network on the microelectrode carbon surface and for enzyme immobilization. Functionalization of the SWNT surface by forming active ester groups was found to considerably enhance SWNT solubility in water with a range from 0.1 to 1.0 mg/mL. The PQQ-dependent GDH immobilized on the surface of the SWNTs exhibited fast heterogeneous electron transfer with a rate constant of 3.6 s~(-1). Moreover, the immobilized PQQ-dependent GDH retained its enzymatic activity for glucose oxidation. A fusion of PQQ-dependent GDH with SWNTs has a great potential for the development of low-cost and reagentless glucose sensors and biofuel cells.
机译：证明了直接生物电催化共价连接到单壁碳纳米管（SWNTs）的吡咯并喹啉醌依赖性葡萄糖脱氢酶（PQQ依赖性GDH）。均质的油墨状SWNT悬浮液既用于在微电极碳表面上创建SWNT网络，又用于酶固定。发现通过形成活性酯基团而使SWNT表面官能化可显着提高SWNT在水中的溶解度，范围为0.1至1.0mg / mL。固定在SWNTs表面的PQQ依赖性GDH表现出快速的异质电子转移，速率常数为3.6 s〜（-1）。此外，固定化的PQQ依赖性GDH保留了其对葡萄糖氧化的酶活性。依赖PQQ的GDH与SWNT的融合具有开发低成本，无试剂葡萄糖传感器和生物燃料电池的巨大潜力。

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