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Preparation of Three-Dimensional Ordered Macroporous Prussian Blue Film Electrode for Glucose Biosensor Application

机译：葡萄糖生物传感器三维有序大孔普鲁士蓝膜电极的制备

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摘要

Ordered 3D interconnected macroporous Prussian blue (PB) films were electrochernically fabricated by using colloidal crystals of polystyrene beads as sacrificial templates. The prepared PB film electrodes have excellent catalytic activity towards the reduction of hydrogen peroxide. The PB structure was further used as functional interface for fabricating an enzyme-based glucose sensor by using surface modification technique based on the electrostatic interactions. The resulted sensor has higher functional density, and larger surface area. The interconnected macroporous structure allows enhanced mass transport. These characteristics of the sensor enable us to detect glucose with high sensitivity. Therefore, the present 3D ordered macroporous film sensor exhibits wide linear detection range towards glucose, acceptable reproducibility and operalional and storage stability. The present approach is promising for the generation of high-enzyme-content thin films with tailored bioactivity.
机译：以聚苯乙烯珠的胶体晶体为牺牲模板，通过电化学法制备了有序的3D互连大孔普鲁士蓝（PB）薄膜。所制备的PB膜电极对还原过氧化氢具有优异的催化活性。通过使用基于静电相互作用的表面修饰技术，PB结构还用作制造基于酶的葡萄糖传感器的功能接口。所得传感器具有较高的功能密度和较大的表面积。相互连接的大孔结构可增强质量传输。传感器的这些特性使我们能够以高灵敏度检测葡萄糖。因此，本发明的3D有序大孔膜传感器对葡萄糖，可接受的再现性以及操作和存储稳定性表现出宽的线性检测范围。本方法有望产生具有定制生物活性的高酶含量的薄膜。

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