• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electroanalysis >Development of a progesterone immunosensor based on a colloidal gold-graphite-teflon composite electrode
【24h】

Development of a progesterone immunosensor based on a colloidal gold-graphite-teflon composite electrode

机译：基于胶体金-石墨-铁氟龙复合电极的孕酮免疫传感器的研制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel amperometric immunosensor for the determination of progesterone, based on direct attachment of antiprogesterone on a gold nanoparticles-modified graphite-Teflon composite electrode is reported. Competitive assay involving progesterone labeled with alkaline phosphatase (AP), and 1-naphtyl phosphate as the enzyme substrate, was employed. The product of the enzyme reaction, 1-naphtol was detected amperometrically at +0.3 V vs. Ag/AgCl. Variables involved in the immunosensor preparation such as the colloidal gold loading and the amount of anti-Prog immobilized, as well as in the immunosensing event (pH, Prog/Prog-AP ratio and volume) were optimized. A calibration plot was obtained for progesterone with a linear range between 0 and 30 ng mL(-1), and a slope value, -1.2 nA ng(-1) mL, which is remarkably higher than that reported for other progesterone biosensors. A good repeatability of the amperometric measurements, RSD = 3.5% (n = 10), and an acceptable fabrication reproducicility, RSD = 8.2% (n = 3), were obtained. ne developed anti-Prog-Au-coll-graphite-Teflon immunosensor was applied to the progesterone determination in milk. Recovery at the 3.5 ng mL(-1) progesterone concentration level was of 101 +/- 6% (n = 7).
机译：报道了一种基于抗孕酮直接附着在金纳米粒子修饰的石墨-特富龙复合电极上的用于测定孕酮的新型安培免疫传感器。采用竞争性测定法，该方法涉及用碱性磷酸酶（AP）标记的孕酮和1-萘基磷酸酯作为酶底物。在相对于Ag / AgCl的+0.3 V电流下安培法检测到了酶反应产物1-萘酚。优化了免疫传感器制备中涉及的变量，例如胶体金负载量和固定的抗Prog量，以及免疫传感事件（pH，Prog / Prog-AP比和体积）。获得了黄体酮的校准图，其线性范围在0到30 ng mL（-1）之间，斜率值为-1.2 nA ng（-1）mL，明显高于其他黄体酮生物传感器的报告值。获得了良好的安培测量重复性，RSD = 3.5％（n = 10），并且可接受的制造再现性RSD = 8.2％（n = 3）。新开发的抗Prog-Au-coll-石墨-Teflon免疫传感器用于牛奶中孕酮的测定。在3.5 ng mL（-1）孕酮浓度下的回收率为101 +/- 6％（n = 7）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号