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首页> 外文期刊>Electroanalysis >Biosensor based on self-assembling glucose oxidase and dendrimer-encapsulated Pt nanoparticles on carbon nanotubes for glucose detection
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Biosensor based on self-assembling glucose oxidase and dendrimer-encapsulated Pt nanoparticles on carbon nanotubes for glucose detection

机译：基于自组装葡萄糖氧化酶和碳纳米管上树枝状聚合物包裹的Pt纳米颗粒的生物传感器用于葡萄糖检测

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摘要

A novel amperometric glucose biosensor based on layer-by-layer (LbL) electrostatic adsorption of glucose oxidase (GOx) and dendrimer-encapsulated Pt nanoparticles (Pt-DENs) on multiwalled carbon nanotubes (CNTs) was described. Anionic GOx was immobilized on the negatively charged CNTs surface by alternatively assembling a cationic Pt-DENs layer and an anionic GOx layer. Transmission electron microscopy images and zeta-potentials proved the formation of layer-by-layer nanostructures on carboxyl-functionalized CNTs. LbL technique provided a favorable microenvironment to keep the bioactivity of GOx and prevent enzyme molecule leakage. The excellent electrocatalytic activity of CNTs and Pt-DENs toward H2O2 and special three-dimensional structure of the enzyme electrode resulted in good characteristics such as a low detection limit of 2.5 mu M, a wide linear range of 5 mu M - 0.65 mM, a short response time (within 5 s), and high sensitivity (30.64 mu A mM(-1) cm(-2)) and stability (80% remains after 30 days).
机译：描述了一种新颖的电流型葡萄糖生物传感器，该传感器基于葡萄糖氧化酶（GOx）和树状聚合物包封的Pt纳米颗粒（Pt-DENs）在多壁碳纳米管（CNTs）上的逐层（LbL）静电吸附。通过交替组装阳离子Pt-DENs层和阴离子GOx层，将阴离子GOx固定在带负电荷的CNT表面上。透射电子显微镜图像和ζ电位证明了在羧基官能化的CNT上形成逐层纳米结构。 LbL技术提供了良好的微环境，可以保持GOx的生物活性并防止酶分子泄漏。 CNT和Pt-DENs对H2O2的出色的电催化活性以及酶电极的特殊三维结构导致了良好的特性，如2.5μM的低检测限，5μM-0.65 mM的宽线性范围，响应时间短（在5 s之内），灵敏度高（30.64μA mM（-1）cm（-2））和稳定性（30天后仍保持80％）。

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