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Direct bioelectrocatalysis by NADP-reducing hydrogenase from Pyrococcus furiosus

机译:激烈热球菌NADP还原氢酶的直接生物电催化

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摘要

The direct bioelectrocatalysis by an NAD(P)-reducing hydrogenase is reported for the first time. In contrast to previous attempts to involve similar enzymes in bioelectrocatalysis [1-4], which were in fact unsuccessful, in our report an effective eleetrocatalysis by Pyrococcus fariosus hydrogenase is convincingly shown by (i) achievement of the hydrogen equilibrium potential and (ii) a high current of hydrogen oxidation (0.3 mA cm(-2) at 100 mV overpotential and at 75 degrees C). The latter is just a few times lower compared to enzyme electrodes based on NAD(P)independent hydrogenases.
机译:首次报道了NAD(P)还原氢酶的直接生物电催化作用。与以前尝试将类似酶参与生物电催化[1-4]的尝试相反,后者实际上是不成功的,在我们的报告中,通过(i)实现氢平衡电位和(ii)证明了火球热球菌氢化酶的有效电催化作用。氢氧化的高电流(在100 mV超电势和75摄氏度下为0.3 mA cm(-2))。与基于NAD(P)的不依赖氢化酶的酶电极相比,后者仅低几倍。

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