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首页> 外文期刊>Electroanalysis >Direct electrochemistry of cytochrome P450 reductases in surfactant and polyion films
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Direct electrochemistry of cytochrome P450 reductases in surfactant and polyion films

机译：表面活性剂和聚离子膜中细胞色素P450还原酶的直接电化学

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NADPH-cytochrome P450 reductase (CPR) serves as electron donor for cytochrome P450 catalyzed monooxygenase reactions utilizing flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) as electron transfer cofactors. Here, stable films of human and rabbit CPRs with didodecyldimethylammonium bromide (DDAB), dimyristoylphosphatidyl choline (DMPC), and poly(diallyidimethylammonium) (PDDA) were made on pyrolytic graphite (PG) electrodes for comparative structural and electrochemical studies. CD and UV-VIS absorbance spectra suggested that near native CPR conformation is retained in PDDA films, and some conformational changes occur in DMPC or DDAB films. Cyclic voltammetry of these films gave quasireversible pairs of peaks at average formal potential -0.246 +/- 0.008 V vs. NHE. In human CPR-DDAB (H-CPR-DDAB), a second pair of peaks at +0.317 V vs. NHE was found that depended strongly on identity of buffer and salt. Excepting H-CPR in DDAB, films showed similar voltammetry, formal potentials, and k(s) values. While CPR-PDDA films had near native CPR structures, electrochemical parameters did not differ significantly from CPR-DMPC films. The relative independence of film voltammetry from the influence of film materials for CPRs is in contrast with heme iron proteins that, while retaining near native structures, have formal potentials that depend significantly on identity of the film material.
机译：NADPH-细胞色素P450还原酶（CPR）作为电子供体，利用黄素单核苷酸（FMN）和黄素腺嘌呤二核苷酸（FAD）作为电子转移辅助因子，用于细胞色素P450催化的单加氧酶反应。在这里，在热解石墨（PG）电极上制作了具有十二烷基二甲基溴化铵（DDAB），二甲基扁豆酰磷脂酰胆碱（DMPC）和聚（二烯丙基二甲基铵）（PDDA）的人和兔子CPR的稳定膜，用于比较结构和电化学研究。 CD和UV-VIS吸收光谱表明，PDDA膜中保留了接近天然的CPR构象，而DMPC或DDAB膜中发生了一些构象变化。这些膜的循环伏安法给出了相对于NHE的平均形式电势-0.246 +/- 0.008 V的准可逆峰对。在人CPR-DDAB（H-CPR-DDAB）中，发现在+0.317 V vs. NHE的第二对峰强烈依赖于缓冲液和盐的同一性。除了DDAB中的H-CPR，薄膜显示出相似的伏安法，形式势和k（s）值。尽管CPR-PDDA膜具有接近天然的CPR结构，但电化学参数与CPR-DMPC膜没有显着差异。膜伏安法相对于膜材料对CPR的影响是相对独立的，与血红素铁蛋白形成对比，而血红素铁蛋白虽然保留了天然结构，但形式势却很大程度上取决于膜材料的特性。

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