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Comparison of DNA hybridization at rotating and heated gold disk electrodes

机译：旋转和加热金盘电极上DNA杂交的比较

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摘要

This paper reports about the influence of temperature, hybridization time and convection upon the detection of osmium tetroxide bipyridine-labeled target oligonucleotides at rotating gold disk (RDE) and heated low temperature co-fired ceramics (LTCC) gold disk electrodes. We used mixed self-assembled monolayers of hexathiol-linked probe oligonucleotides and mercaptohexanol on the gold surface of the electrodes for the hybridization detection of the labeled targets by means of square-wave voltammetry. Due to protective strands, the osmium tetroxide-modified target strands were still able to hybridize with the immobilized probe strands. The hybridization of such osmium tetroxide bipyridine-modified target strands with thiol-linked probe strands immobilized on gold yielded large reversible square-wave-voltammetric signals. Rotation speed and, hence, mass transport due to convection has only marginal effects. On the other hand, temperature affects greatly the hybridization step as indicated by both heated LTCC electrode in cold and RDE in warm hybridization solution. Calculated detection limits of 3.6 and 3.1 nM targets at the RDE and the LTCC electrode, respectively, have been almost the same at both types of electrodes. Applying an appropriate temperature during hybridization is more important than mechanically enhanced mass transport.
机译：本文报道了温度，杂交时间和对流对旋转金盘（RDE）和加热的低温共烧陶瓷（LTCC）金盘电极上四氧化os联吡啶标记的目标寡核苷酸检测的影响。我们在电极的金表面上使用了六硫醇连接的探针寡核苷酸和巯基己醇的混合自组装单分子层，用于通过方波伏安法杂交检测标记的靶标。由于保护性链，四氧化修饰的靶链仍然能够与固定的探针链杂交。这种四氧化联吡啶修饰的靶标链与固定在金上的硫醇连接的探针链的杂交产生了大的可逆方波伏安信号。旋转速度以及对流引起的质量传输仅具有边际效应。另一方面，温度会极大地影响杂交步骤，冷的加热LTCC电极和热的杂交溶液中的RDE都表明温度。在两种类型的电极上，分别在RDE和LTCC电极上计算出的3.6和3.1 nM目标检测限几乎相同。在杂交过程中施加适当的温度比机械增强的质量传递更为重要。

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