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Direct electron transfer reaction of ascorbate oxidase immobilized by a self-assembled monolayer and polymer membrane combined system

机译：自组装单层与聚合物膜结合体系固定化的抗坏血酸氧化酶的直接电子转移反应

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摘要

This paper reports a novel enzyme-immobilization method for the direct electron transfer (DET) reaction of ascorbate oxidase from Acremonium sp. HI-25 (ASOM). ASOM was adsorbed onto a gold electrode modified with a self-assembled monolayer (SAM) of alkanethiol derivatives and immobilized by a cationic polymer membrane and anionic omega-carboxyalkanethiol combined system. The redox responses of the immobilized ASOM were investigated by cyclic voltammetry. We found that the DET reaction of ASOM was facilitated by this novel immobilization. On the other hand, the redox responses of poly(ethylene oxide) (PEO)-modified ASOMs were also investigated. ASOM was modified with two types of PEO which possess straight chain-shaped (PEO2000) or comb-shaped conformation (PM67)As a result, the DET reactions of PEO-modified ASOMs were also facilitated by this immobilization method. We concluded that this immobilization method is effective for promoting the DET reaction of ASOMs.
机译：本文报道了一种新的酶固定化方法，用于Acremonium sp。中抗坏血酸氧化酶的直接电子转移（DET）反应。 HI-25（ASOM）。将ASOM吸附到用链烷硫醇衍生物的自组装单层（SAM）修饰的金电极上，并通过阳离子聚合物膜和阴离子ω-羧基链烷硫醇组合系统固定化。通过循环伏安法研究了固定的ASOM的氧化还原反应。我们发现这种新型固定化促进了ASOM的DET反应。另一方面，还研究了聚环氧乙烷（PEO）改性的ASOM的氧化还原响应。用两种具有直链形（PEO2000）或梳形构型（PM67）的PEO对ASOM进行了改性。结果，这种固定化方法也促进了PEO改性的ASOM的DET反应。我们得出的结论是，这种固定方法对于促进ASOM的DET反应是有效的。

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