...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Electroanalysis >Ultrasensitive Electrochemical Immunosensor for Detection of Tumor Necrosis Factor-alpha Based on Functionalized MWCNT-Gold Nanoparticle/Ionic Liquid Nanocomposite
【24h】

Ultrasensitive Electrochemical Immunosensor for Detection of Tumor Necrosis Factor-alpha Based on Functionalized MWCNT-Gold Nanoparticle/Ionic Liquid Nanocomposite

机译：基于功能化MWCNT-金纳米粒子/离子液体纳米复合材料的肿瘤敏感因子α超灵敏电化学免疫传感器

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel and highly sensitive electrochemical immunosensor was developed for the detection of protein biomarker tumor necrosis factor-alpha (TNF-alpha) based on immobilization of TNF-alpha-antibody (anti-TNF-alpha) onto robust nanocomposite containing gold nanoparticles (AuNP), multiwalled carbon nanotubes (MWCNTs) and ionic liquid (1-buthyl-3-methylimidazolium bis (trifluoromethyl sulfonyl) imide). Functionalized MWCNT-gold nanoparticle was produced by one-step synthesis based on the direct redox reaction. The electrochemical properties of nanocomposite were characterized by electrochemical impedance spectroscopy and cyclic voltammetry. The anti-TNF-alpha was immobilized or entrapped in the nanocomposite and used in a sandwich type complex immunoassay with anti-TNF-alpha labeled with horseradish peroxidase as secondary antibody. Under optimum conditions, the immunosensor could detect TNF-alpha in a linear range from 6.0 to 100 pgmL(-1) with a low detection limit of 2.0 pgmL(-1). The simple fabrication method, high sensitivity, good reproducibility, stability, as well as acceptable accuracy for TNF-alpha detection in human serum samples are the main advantages of this immunosensor, which might have broad applications in protein diagnostics and bioassay.
机译：基于将TNF-α-抗体（anti-TNF-alpha）固定在含有金纳​​米颗粒（AuNP）的坚固的纳米复合材料上，开发了一种新型的高度灵敏的电化学免疫传感器，用于检测蛋白质生物标志物肿瘤坏死因子-α（TNF-α）。 ，多壁碳纳米管（MWCNT）和离子液体（1-丁基-3-甲基咪唑双（三氟甲基磺酰基）酰亚胺）。通过基于直接氧化还原反应的一步合成来生产功能化的MWCNT-金纳米颗粒。通过电化学阻抗谱和循环伏安法表征了纳米复合材料的电化学性能。将抗TNF-α固定或截留在纳米复合物中，并用于夹心型复合免疫测定中，其中以辣根过氧化物酶标记的抗TNF-α为二抗。在最佳条件下，该免疫传感器可以在6.0到100 pgmL（-1）的线性范围内检测TNF-α，检测限低至2.0 pgmL（-1）。这种免疫传感器的主要优点是：制备方法简单，灵敏度高，重现性好，稳定性好以及在人血清样品中检测TNF-α的可接受的准确性，在蛋白质诊断和生物测定中可能具有广泛的应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号