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首页> 外文期刊>Electroanalysis >Electrochemical Magnetoimmunosensor for Progesterone Receptor Determination. Application to the Simultaneous Detection of Estrogen and Progesterone Breast-cancer Related Receptors in Raw Cell Lysates.
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Electrochemical Magnetoimmunosensor for Progesterone Receptor Determination. Application to the Simultaneous Detection of Estrogen and Progesterone Breast-cancer Related Receptors in Raw Cell Lysates.

机译：用于孕酮受体测定的电化学磁免疫传感器。在同时检测原始细胞裂解物中雌激素和孕激素乳腺癌相关受体中的应用。

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摘要

An electrochemical magnetoimmunosensor for the determination of progesterone receptor (PR) making use of functionalized magnetic microbeads (MBs), a sandwich-type immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs) was described for the first time in this work. This selective and sensitive magnetoimmunosensor, with a detection limit of 22pgmL(-1), was successfully applied to the determination of PR in raw cell lysates with just a dilution as sample treatment and exhibiting no matrix effect. The advantageous performance in terms of simplicity and assay time over commercial ELISAs makes it competitive with conventional strategies providing rapidly quantitative and reliable results on this biomarker currently used in the clinical practice for diagnosis, follow-up and monitoring of metastatic breast cancer. In addition, a dual magnetoimmunosensor using screen-printed dual carbon electrode (SPdCE) has been implemented for the simultaneous determination of PR and estrogen receptor (ER) in cell lysates. This dual receptor determination allows a more accurate diagnosis enabling a more efficient treatment. The usefulness of this dual magnetoimmunosensing platform was demonstrated by discriminating raw lysates of two types of cancer cells (MCF-7 and MDA-MB-436) with significantly different PR/ER expression levels.
机译：这项工作中首次描述了利用功能化磁性微珠（MBs）来测定孕激素受体（PR）的电化学磁免疫传感器，夹心型免疫测定和一次性丝网印刷碳电极（SPCE）的安培检测。这种选择性灵敏的磁免疫传感器的检出限为22pgmL（-1），已成功地用于原细胞裂解液中PR的测定，只需稀释即可作为样品处理，并且没有基质效应。就商业化ELISA而言，在简便性和检测时间方面，其优越的性能使其可与常规策略竞争，从而可对目前用于临床实践中的转移性乳腺癌的诊断，随访和监测的这种生物标记物提供快速定量和可靠的结果。此外，已经实现了使用丝网印刷双碳电极（SPdCE）的双磁免疫传感器，用于同时测定细胞裂解物中的PR和雌激素受体（ER）。该双重受体确定允许更准确的诊断，从而实现更有效的治疗。通过区分PR / ER表达水平明显不同的两种类型的癌细胞（MCF-7和MDA-MB-436）的原始裂解物，证明了这种双重磁免疫传感平台的实用性。

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