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Enzyme Based Electrochemical Biosensor for Ethanolamine

机译：乙醇胺的基于酶的电化学生物传感器

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Enzyme based electrochemical detection method developed for chemical toxicant ethanolamine (EA). Monoamine oxidase A (MAO-A) enzyme was used for the oxidation of EA. A direct electron transfer from the electrode to EA without any mediator with the help of MAO-A enzyme was attained and this confirms the application of this methodology for the development of third generation biosensor for EA sensing well below the IDLH (30 ppm) value of EA. Moreover, heterogeneous rate constant (0.021s(-1)) and the number of electron involved (5.2) were deduced for EA in PBS buffer. The calibration plot showed linearity 2.02x10(-4) M to 10.10x10(-4) M of EA in PBS buffer with detection limit 4.1 ppm. The modified electrodes are characterized by Raman and Electrochemical impedance spectroscopy (EIS) techniques. The outcome of this work indicates about the utility of this methodology for the sensing of EA in the environment if it is present as well as to degrade EA into other compounds without using any indicator or mediator.
机译：针对化学毒物乙醇胺（EA）开发了基于酶的电化学检测方法。单胺氧化酶A（MAO-A）酶用于EA的氧化。在没有任何介体的情况下，借助于MAO-A酶，可将电子直接从电极转移到EA，这证实了该方法在开发第三代生物传感器中的应用，该传感器可远低于IDLH（30 ppm）的IDLH（30 ppm）值。 EA。此外，推导了PBS缓冲液中EA的异质速率常数（0.021s（-1））和涉及的电子数（5.2）。校正图显示在PBS缓冲液中EA的线性度为2.02x10（-4）M到10.10x10（-4）M到EA，检测极限为4.1 ppm。改性电极通过拉曼和电化学阻抗谱（EIS）技术进行表征。这项工作的结果表明，该方法可用于检测环境中的EA（如果存在）以及在不使用任何指示剂或介体的情况下将EA降解为其他化合物。

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