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首页> 外文期刊>Electroanalysis >Electrochemical quartz crystal microbalance studies on cytochrome c/polyelectrolyte multilayer assemblies on gold electrodes
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Electrochemical quartz crystal microbalance studies on cytochrome c/polyelectrolyte multilayer assemblies on gold electrodes

机译：金电极上细胞色素c /聚电解质多层组件的电化学石英晶体微量天平研究

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摘要

Polyelectrolyte multilayer assemblies containing proteins are of interest for applications such as sensors, bioreactors, and bioelectronics. A multilayer electrode was built up by the layer-by-layer strategy consisting of alternating layers of cytochrome c and poly(aniline sulfonic acid). The electrode showed a linear increase of redox active protein with the number of deposited layers. The principle of electrode preparation was transferred from needle electrodes to planar surfaces in order to further the understanding of electron transfer through the layer assembly by means of electrochemical quartz crystal microbalance studies. The deposition process was followed on-line by detection of the frequency shift of the crystals and was found to be rather fast (minutes). The total mass deposited was found to correlate well with the electrochemical response of the immobilized cyt.c. Furthermore, the influence of the polyelectrolyte was investigated by addition of PSS to the PASA solution. The strong interaction of the former polyelectrolyte seemed to hinder the electron transfer although a multilayer formation was proved. Dilution of the protein solution with redox inactive apo-cyt.c led to a strong decrease of the voltammetric signal, well beyond the percentage of apo-cyt.c inside the assembly. Thus, arguments for an electron transfer via protein-protein interaction were found.
机译：包含蛋白质的聚电解质多层组件对于诸如传感器，生物反应器和生物电子学的应用是令人感兴趣的。通过逐层策略构建多层电极，该策略由细胞色素c和聚苯胺磺酸的交替层组成。电极显示氧化还原活性蛋白随沉积层数线性增加。电极制备的原理已从针状电极转移到平坦表面，以通过电化学石英晶体微量天平研究进一步了解电子通过层组件的转移。通过检测晶体的频移，在线跟踪沉积过程，发现沉积过程相当快（数分钟）。发现沉积的总质量与固定化cyt.c的电化学反应良好相关。此外，通过将PSS添加到PASA溶液中研究了聚电解质的影响。尽管已证明形成多层，但前一种聚电解质的强相互作用似乎阻碍了电子转移。用氧化还原非活性载脂蛋白-cyt.c稀释蛋白质溶液会导致伏安信号的强烈下降，远远超过装配体内载脂蛋白-cyt.c的百分比。因此，找到了通过蛋白质-蛋白质相互作用进行电子转移的观点。

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