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Multiwall carbon nanotube (MWCNT) based electrochemical biosensors for mediatorless detection of putrescine

机译:基于多壁碳纳米管(MWCNT)的电化学生物传感器,用于腐胺的无介质检测

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摘要

gamma-Aminopropyltriethoxysilane (APTES)-induced solubilization of multi-wall carbon nanotube CNTs allowed for the modification of electrode surfaces. APTES also served as an immobilization matrix for putrescine oxidase (POx) to construct an amperometric biosensor. Although CNTs modified by APTES acted as semiconductors to reduce the exposed sensing surface, we reasoned that nanoscale "dendrites" of CNTS modified by APTES formed a network and projected outwards from the electrode surface and acted like bundled ultra-microelectrodes that allowed access to the active site and facilitated direct electron transfer to the immobilized enzyme. Our biosensor was able to efficiently monitor direct electroactivity of POx at the electrode surface. The putrescine biosensor prepared using the modified glassy carbon electrode exhibited current response within 10 s with a detection limit of 500 nM.
机译:γ-氨基丙基三乙氧基硅烷(APTES)诱导的多壁碳纳米管CNT增溶可修饰电极表面。 APTES还用作腐胺氧化酶(POx)的固定基质,以构建电流型生物传感器。尽管APTES修饰的CNT充当半导体以减少暴露的传感表面,但我们认为APTES修饰的CNTS的纳米级“枝晶”形成了一个网络并从电极表面向外突出,并且像束状超微电极一样起作用,可以进入活性物质。定位并促进直接电子转移至固定化酶。我们的生物传感器能够有效监控电极表面POx的直接电活性。使用修饰的玻碳电极制备的腐胺生物传感器在10 s内表现出电流响应,检测极限为500 nM。

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