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首页> 外文期刊>Electroanalysis >Construction of L-Lysine Sensor by Layer-by-Layer Adsorption of L-Lysine 6-Dehydrogenase and Ferrocene-Labeled High Molecular Weight Coenzyme Derivative on Gold Electrode
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Construction of L-Lysine Sensor by Layer-by-Layer Adsorption of L-Lysine 6-Dehydrogenase and Ferrocene-Labeled High Molecular Weight Coenzyme Derivative on Gold Electrode

机译：金电极上层层吸附L-赖氨酸6-脱氢酶和二茂铁标记的高分子量辅酶衍生物构建L-赖氨酸传感器

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摘要

A ferrocene-labeled high molecular weight coenzyme derivative (PEI-Fc-NAD) and a thermostable NAD-dependent L-lysine 6-dehydrogenase (LysDH) from thermophile Geobacillus stearothermophilus were used to fabricate a reagentless L-lysine sensor. Both LysDH and PEI-Fc-NAD were immobilized oil the surface of a gold electrode by consecutive layer-by-layer adsorption (LBL) technique. By the simple LBL method, the reagentless L-lysine sensor, with co-immobilization of the mediator, coenzyme, and enzyme was obtained, which exhibited current response to L-lysine without the addition of native coenzyme to the analysis system. The amperometric response of the sensor was dependent on the applied potential, bilayer number of PEI-Fc-NAD/LysDH, and substrate concentration. A linear current response, proportional to L-lysine concentration in the range of 1-120 mM was observed. The response of the sensor to L-lysine was decreased by 30% from the original activity after one month storage.
机译：二茂铁标记的高分子量辅酶衍生物（PEI-Fc-NAD）和嗜热嗜热地热嗜热芽孢杆菌的热稳定NAD依赖性L-赖氨酸6-脱氢酶（LysDH）用于制造无试剂L-赖氨酸传感器。通过连续的逐层吸附（LBL）技术将LysDH和PEI-Fc-NAD固定在金电极的表面。通过简单的LBL方法，获得了无试剂的L-赖氨酸传感器，并同时固定了介体，辅酶和酶，该传感器在不向分析系统中添加天然辅酶的情况下，表现出对L-赖氨酸的电流响应。传感器的安培响应取决于施加的电势，PEI-Fc-NAD / LysDH的双层数和底物浓度。观察到线性电流响应，与L-赖氨酸浓度成比例，范围为1-120 mM。储存一个月后，传感器对L-赖氨酸的反应比原始活性降低了30％。

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