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首页> 外文期刊>Electroanalysis >Electrochemical immunoassay for free prostate specific antigen (f-PSA) using magnetic beads
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Electrochemical immunoassay for free prostate specific antigen (f-PSA) using magnetic beads

机译：使用磁珠的游离前列腺特异性抗原（f-PSA）的电化学免疫测定

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Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of <0.1 ng mL(-1) f-PSA and a linear range of 0 to 1 ng mL(-1) f-PSA.
机译：前列腺特异性抗原（PSA）是前列腺癌的重要标志。它以游离（f-PSA）和复杂形式存在于人血中。这两种形式统称为总PSA（t-PSA）。两种形式的估计对于预测恶性肿瘤至关重要。在这项研究中，我们报告了一种使用三电极丝网印刷传感器上的磁珠对f-PSA进行电化学检测的独特而有效的技术。在比色皿中进行磁珠酶联免疫吸附测定（ELISA）。免疫测定后，通过磁选机回收磁珠并转移到3电极组件的工作电极上。通过添加酶底物来确定安培响应，即对珠子上残留的酶活性的量的测量，并因此测量溶液中分析物的浓度。该设备的检测极限为<0.1 ng mL（-1）f-PSA，线性范围为0到1 ng mL（-1）f-PSA。

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