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首页> 外文期刊>Electroanalysis >An amperometric biosensor for trans-resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from Brassica napus
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An amperometric biosensor for trans-resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from Brassica napus

机译：通过用甘蓝型油菜的过氧化物酶碱性同工酶修饰的碳糊电极测定水溶液中反式白藜芦醇的安培生物传感器

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The catalytic properties of peroxidase basic isoenzymes (PBI's) from Brassica napus towards trans-resveratrol (t-Res) oxidation were demonstrated by the first time by conventional UV-visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBI's to determine t-Res is also proposed by the first time. The method employs a dialysis membrane covered, PBI's entrapped and ferrocene (Fc)-embedded carbon paste electrode (PBI's-Fc-CP) and is based on the fact that the decreased amount of H2O2 produced by the action of PBI's is proportional to the oxidised amount of t-Res in the solution. Comparative amperometric experiments showed that, in spite of PBI's activity was much lower than commercial horseradish peroxidase (HRP) activity, t-Res was a much better substrate for PBI's biosensors than those biosensors constructed by using HRP. The PBI's-Fe-CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t-Res concentration (Ct-Res) range from 1 x 10(-6) to 2.5 x 10(-5) M, with a sensibility of (2.31 +/- 0.05) x 10(6) nA M-1. The lowest Ct-Res value measured experimentally for a signal to noise ratio of 3:1 was 0.83 mu M.
机译：通过传统的紫外-可见光谱测量首次证明了来自甘蓝型油菜的过氧化物酶碱性同工酶（PBI's）对反式白藜芦醇（t-Res）氧化的催化性能。研究了不同实验条件下的酶促反应速率，确定了动力学参数。还首次提出了基于甘蓝型油菜PBI的安培生物传感器来确定t-Res。该方法采用覆盖有透析膜的PBI包裹并嵌入二茂铁（Fc）的碳糊电极（PBI's-Fc-CP），并且基于以下事实：PBI的作用所产生的H2O2减少量与氧化的量成正比。溶液中的t-Res数量。对比安培实验表明，尽管PBI的活性远低于商业辣根过氧化物酶（HRP）活性，但t-Res是PBI生物传感器比使用HRP构建的生物传感器更好的基质。 PBI-Fe-CP生物传感器在至少二十天内显示出非常好的稳定性。重现性和重复性分别为4.5％和8.3％，显示出良好的生物传感器性能。校正曲线在1 x 10（-6）至2.5 x 10（-5）M的t-Res浓度（Ct-Res）范围内呈线性，灵敏度为（2.31 +/- 0.05）x 10（6） ）nA M-1。实验测得的信噪比为3：1的最低Ct-Res值为0.83μM。

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