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首页> 外文期刊>Electroanalysis >Electrochemical evaluation of the interaction between avidin and biotin at biotinylated polypyrrole electrode using a redox marker
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Electrochemical evaluation of the interaction between avidin and biotin at biotinylated polypyrrole electrode using a redox marker

机译：使用氧化还原标记物对生物素化聚吡咯电极上抗生物素蛋白和生物素之间相互作用的电化学评估

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The interaction between avidin and biotin was evaluated electrochemically by monitoring the change in the electrode response of redox markers. Biotin was immobilized on the electrode surface by means of the electrochemical polymerization of biotinylated pyrrole and pyrrole. When avidin was introduced onto the biotinylated polypyrrole electrode surface, the large change in the electrode response of the redox marker was detected. The fact that the change in the electrode response of a marker ion could be attributed to the electrostatic interaction between avidin on the electrode surface and the redox marker ion present in a solution was verified by replacing avidin with NutrAvidin. At a pH lower than the isoelectric point of avidin, the electrode response of ferrocyanide as an anionic marker ion increased linearly within the range of 5.0 x 10(-9) -3.0 x 10(-8) M avidin. The relative standard deviation at 1.5 x 10(-8) M avidin was about 5.4% (n = 5). The detection of biotin was also performed using a competitive reaction between biotin in solution and biotin that had been immobilized on the electrode surface in the form of the biotinylated polypyrrole. [References: 26]
机译：通过监测氧化还原标记物电极响应的变化，用电化学方法评估了抗生物素蛋白和生物素之间的相互作用。通过生物素化的吡咯和吡咯的电化学聚合将生物素固定在电极表面上。当将抗生物素蛋白引入生物素化的聚吡咯电极表面时，检测到氧化还原标记物的电极响应发生较大变化。通过用NutrAvidin代替抗生物素蛋白，可以证实标志物离子的电极响应变化可能归因于电极表面上抗生物素蛋白与溶液中存在的氧化还原标志物离子之间的静电相互作用。在低于亲和素等电点的pH值下，作为阴离子标记离子的亚铁氰化物的电极响应在5.0 x 10（-9）-3.0 x 10（-8）M亲和素范围内线性增加。 1.5 x 10（-8）M抗生物素蛋白的相对标准偏差约为5.4％（n = 5）。还使用溶液中生物素与已经以生物素化聚吡咯形式固定在电极表面上的生物素之间的竞争性反应来进行生物素的检测。 [参考：26]

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