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首页> 外文期刊>Electroanalysis >On-line monolithic enzyme reactor fabricated by sol-gel process for elimination of ascorbic acid while monitoring dopamine
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On-line monolithic enzyme reactor fabricated by sol-gel process for elimination of ascorbic acid while monitoring dopamine

机译：通过溶胶-凝胶工艺制造的在线整体式酶反应器，用于在监测多巴胺的同时消除抗坏血酸

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Catecholamine, a well-known neurotransmitter, is released to restore heart function after the occurrence of cardiac ischemia. Endogenous ascorbic acid interferes considerably with the monitoring of neurotransmitters such as catecholamine and glutamate. In this work we have successfully developed a nanoliter volume monolithic enzyme reactor and integrated it with a carbon film electrode for monitoring dopamine in order to scavenge such electroactive interferents as ascorbic acid. A monolithic silica support prepared by the sol-gel process has a large through-pore structure and a nanoporous surface. The low back pressure resulting from the large though-pore structure makes it possible to immobilize the enzyme and introduce fluid into the flow monitoring system by using a microsyringe pump. Ascorbate oxidase (AOx) was immobilized on the nanoporous surface of a monolithic matrix by physical adsorption and used for converting the ascorbic acid into its electrochemically inert form, dehydroascorbic acid, while monitoring dopamine. We showed that more than 99.8% of 100 muM of ascorbic acid could be converted to its oxidized form in the monolithic enzyme reactor. We succeeded in monitoring dopamine at a concentration of less than 100 nM in the presence of 50 muM of ascorbic acid. Therefore, this work demonstrated that a monolithic silica support offers excellent potential in regards to realizing a highly selective enzymatic reactor for biosensors.
机译：心脏缺血发生后，儿茶酚胺（一种众所周知的神经递质）被释放以恢复心脏功能。内源性抗坏血酸极大地干扰了神经递质的监测，例如儿茶酚胺和谷氨酸。在这项工作中，我们成功开发了纳升体积的整体式酶反应器，并将其与用于监测多巴胺的碳膜电极集成在一起，以清除诸如抗坏血酸之类的电活性干扰物。通过溶胶-凝胶法制备的整体式二氧化硅载体具有大的通孔结构和纳米孔表面。大通孔结构所产生的低背压使得可以固定酶，并通过使用微注射器泵将流体引入流量监测系统。通过物理吸附将抗坏血酸氧化酶（AOx）固定在整体基质的纳米孔表面上，并用于在监测多巴胺的同时将抗坏血酸转化为其电化学惰性形式脱氢抗坏血酸。我们显示，在整体式酶反应器中，超过100.MuM的抗坏血酸中有99.8％可以转化为其氧化形式。我们成功地在50μM抗坏血酸的存在下监测了浓度低于100 nM的多巴胺。因此，这项工作证明了整体式二氧化硅载体在实现用于生物传感器的高选择性酶反应器方面具有极好的潜力。

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