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Lactate Dehydrogenase Biosensor Based on an Hybrid Carbon Nanotube-Conducting Polymer Modified Electrode

机译：基于杂化碳纳米管导电聚合物修饰电极的乳酸脱氢酶生物传感器

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摘要

A lactate biosensor constructed by immobilization of the enzyme lactate dehydrogenase (LDH) on a glassy carbon electrode modified with a hybrid material composed of the conducting polymer poly (3-methylthiophene) (P3 MT) electrodeposited on the electrode surface, and multiwalled carbon nanotubes (MWCNTs), is reported. The excellent characteristics of the electrode modified with the hybrid material toward the electrochemical oxidation of NADH allowed the construction of a robust LDH electrochemical biosensor able to operate at a working potential as low as +300 mV without the need for redox mediator. Experimental variables affecting the performance of the biosensor: amount of LDH immobilized on the modified electrode surface, buffer composition and working pH value, MWCNTs loading in the hybrid material and NAD+ concentration were optimized. Under the optimized conditions, a linear calibration graph for lactate was obtained over the 1.0 x 10(-6) - 5.0 x 10(-4) M (r=0.9993) concentration range, with a detection limit of 5.6 x 10(-7) M. The biosensor design showed good repeatability of the measurements, good reproducibility in the inter-biosensor assays and a good selectivity against other organic acids, especially when the biosensor is coated with a Nafion film.
机译：通过将乳酸脱氢酶（LDH）固定在玻碳电极上构建的乳酸生物传感器，玻碳电极由杂化材料修饰而成，该杂化材料由电沉积在电极表面的导电聚合物聚（3-甲基噻吩）（P3 MT）和多壁碳纳米管（ MWCNTs）。用杂化材料修饰的电极对NADH进行电化学氧化的优异特性使得能够构建坚固的LDH电化学生物传感器，该传感器能够在低至+300 mV的工作电位下运行，而无需氧化还原介体。影响生物传感器性能的实验变量：固定在修饰电极表面上的LDH量，缓冲液组成和工作pH值，杂化材料中的MWCNT负载和NAD +浓度均得到优化。在最佳条件下，在1.0 x 10（-6）-5.0 x 10（-4）M（r = 0.9993）浓度范围内获得了乳酸的线性校准图，检出限为5.6 x 10（-7） M.生物传感器的设计显示出良好的测量可重复性，生物传感器间测定的可重复性以及对其他有机酸的良好选择性，特别是当生物传感器涂有Nafion膜时。

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