Use of Adsorptive Transfer Stripping Voltammetry for Analyzing Variations of Cytosine Methylation in DNA

Ioannou A; Alexiadou D; Kouidou S; Girousi S; Voulgaropoulos A

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Use of Adsorptive Transfer Stripping Voltammetry for Analyzing Variations of Cytosine Methylation in DNA

机译：吸附转移溶出伏安法用于分析DNA中胞嘧啶甲基化的变化

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The electrochemical behavior and thermal stability of double stranded oligonucleotides containing 5-methyl-cytosine and 7-deaza-guanosine as nucleotide analogues, as well as of Jurkat genomic DNAs methylated to different degree were studied by ACV and SWV and by thernal denaturation analysis. ACV and SWV combined with thermal denaturation analysis of the natural and modified oligonucleotides gave information regarding the presence of methylation and the concomitant conformational changes. ACV and SWV of Jurkat DNA mixtures methylated to different degrees revealed a decrease of the peak heights with increasing methylation, indicating an increase of structural rigidity, in agreement with the thermal denaturation data. These results verify the, possibly local, conformational changes introduced by DNA methylation. The results obtained in all cases were reproducible.

机译：通过ACV和SWV以及热变性分析研究了以5-甲基胞嘧啶和7-脱氮-鸟苷为核苷酸类似物的双链寡核苷酸以及甲基化的Jurkat基因组DNA的化学行为和热稳定性。 ACV和SWV结合天然寡核苷酸和修饰寡核苷酸的热变性分析可得出有关甲基化的存在以及随之而来的构象变化的信息。甲基化程度不同的Jurkat DNA混合物的ACV和SWV显示，随着甲基化程度的增加，峰高降低，这表明结构刚度增加，与热变性数据一致。这些结果证实了DNA甲基化可能引起的局部构象变化。在所有情况下获得的结果都是可重复的。

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《Electroanalysis》 |2009年第24期|共8页
作者
Ioannou A; Alexiadou D; Kouidou S; Girousi S; Voulgaropoulos A;
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正文语种 eng
中图分类电化学、电解、磁化学;
关键词
DNA methylation; Electroanalysis; Melting analysis; Oligonucleotides; 5-Aza-2 '-deoxycytidine;

机译：DNA甲基化;电泳分析;融合分析;寡核苷酸;5-Aza-2'-脱氧胞苷;

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专利

1. Use of Adsorptive Transfer Stripping Voltammetry for Analyzing Variations of Cytosine Methylation in DNA [J] . Ioannou A, Alexiadou D, Kouidou S, Electroanalysis . 2009,第24期

机译：吸附转移溶出伏安法用于分析DNA中胞嘧啶甲基化的变化
2. Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize [J] . Masarik M., Kizek R., Kramer KJ., Analytical chemistry . 2003,第11期

机译：抗生物素蛋白-生物素技术和吸附转移剥离方波伏安法在转基因抗生物素蛋白玉米中检测DNA杂交和抗生物素蛋白的应用
3. Adsorptive transfer stripping AC voltammetry of DNA complexes with intercalators [J] . Fojta M., Fulneckova J., Kubicarova T., Electroanalysis . 2000,第12期

机译：嵌入剂吸附转移溶出交流伏安法测定DNA配合物。
4. Targeted profiling of 5-(hydroxy)methylcytosine in genomic DNA from human livers: Next-generation sequencing of target enriched DNA reveals unexpectedly high interindividual variability of cytosine methylation and hydroxymethylation [C] . Ivanov Maxim, Ingelman-Sundberg Magnus, Kals Mart, IEEE International Conference on Bioinformatics and Biomedicine . 2014

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5. Developing DNA probes to trap DNA cytosine-5 methyltransferases involved in promoter hypermethylation in cancer. [D] . Shigdel, Uddhav Kumar. 2009

机译：开发DNA探针来捕获参与癌症启动子高甲基化的DNA胞嘧啶-5甲基转移酶。
6. Electrochemically Reduced Graphene Oxide-Based Screen-Printed Electrodes for Total Tetracycline Determination by Adsorptive Transfer Stripping Differential Pulse Voltammetry [O] . Anabela S. Lorenzetti, Tania Sierra, Claudia E. Domini, 2020

机译：吸附转移溶出差分脉冲伏安法测定电化学还原氧化石墨烯基丝网印刷电极用于测定四环素
7. DNA methyltransferase 1, cytosine methylation, and cytosine hydroxymethylation in mammalian mitochondria [O] . Shock, Lisa S., Thakkar, Prashant V., Peterson, Erica J., 2011

机译：哺乳动物线粒体中的DNA甲基转移酶1，胞嘧啶甲基化和胞嘧啶羟甲基化

Use of Adsorptive Transfer Stripping Voltammetry for Analyzing Variations of Cytosine Methylation in DNA

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