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首页> 外文期刊>Electroanalysis >Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate
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Polymeric Membrane Ion-Selective Electrode for Butyrylcholinesterase Based on Controlled Release of Substrate

机译：基于底物控释的丁酰胆碱酯酶聚合膜离子选择电极

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摘要

A butyrylcholine selective polymeric membrane electrode using o-beta-cyclodextrin as ionophore is described as a reagent controlled-release system for reagentless detection of butyrylcholinesterase. Butyrylcholine released across the membrane from inner filling solution of the ion selective electrode is consumed by reaction of the enzyme in sample solution and the concentration of butyrylcholine at the membrane surface can be sensed potentiometrically. The electrode with 0.01 M butyrylcholine in the inner solution yields a potential that varies linearly with butyrylcholinesterase concentration over the range of 0.0075-0.15 U mL(-1) in 0.02 M of pH 7.4 sodium phosphate buffer solution. This approach can also be used to analyze butyrylcholinesterase inhibitors such as organophosphate pesticides. The inhibition percentage of parathion is proportional to its concentration in the range of 0.05-0.5 ng mL(-1) with a detection limit of 0.03 ng mL(-1).
机译：使用邻-β-环糊精作为离子载体的丁酰胆碱选择性聚合物膜电极被描述为用于丁酰胆碱酯酶的无试剂检测的试剂控释系统。从离子选择电极的内部填充溶液跨膜释放的丁酰胆碱被样品溶液中的酶反应消耗，并且可以通过电位法感测膜表面上的丁酰胆碱浓度。内溶液中含有0.01 M丁酰胆碱的电极产生的电位在0.02 M pH 7.4磷酸钠缓冲溶液中的0.0075-0.15 U mL（-1）范围内随丁酰胆碱酯酶浓度线性变化。该方法还可用于分析丁酰胆碱酯酶抑制剂，例如有机磷酸酯农药。对硫磷的抑制百分比与其浓度在0.05-0.5 ng mL（-1）范围内成正比，检出限为0.03 ng mL（-1）。

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