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首页> 外文期刊>Electroanalysis >Direct electrochemistry and bioelectrocatalysis of microperoxidase-11 immobilized on chitosan-graphene nanocomposite
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Direct electrochemistry and bioelectrocatalysis of microperoxidase-11 immobilized on chitosan-graphene nanocomposite

机译:固定在壳聚糖-石墨烯纳米复合材料上的微过氧化物酶-11的直接电化学和生物电催化

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摘要

A bioelectrochemical platform has been constructed for the direct electron transfer and biosensing purposes of microperoxidase-11 (MP-11) immobilized on the chitosan dispersed multilayer graphene nanocomposite. The immobilized MP-11 at the modified gold electrode displays a well-defined and quasireversible redox peaks, with a formal potential of - 0.38 V/SCE in a buffer solution (pH 7.0). MP-11 absorbed on the electrode surface exhibits high electrocatalytic activity toward the reduction of both oxygen and hydrogen peroxide and also shows good analytical performance for the amperometric detection of H2O2 with a linear range from 2.5 to 135 mM. These results indicate the graphene modified electrode might be used as a third generation biosensor for H2O2 detection.
机译:为了固定化在壳聚糖分散的多层石墨烯纳米复合材料上的微过氧化物酶11(MP-11)的直接电子转移和生物传感目的,已经构建了一个生物电化学平台。固定在修饰金电极上的MP-11表现出明确的和准可逆的氧化还原峰,在缓冲溶液(pH 7.0)中的形式电位为-0.38 V / SCE。吸附在电极表面的MP-11表现出对减少氧气和过氧化氢的高电催化活性,并且还显示出用于安培检测H2O2的良好分析性能,线性范围为2.5至135 mM。这些结果表明,石墨烯修饰的电极可以用作第三代生物传感器检测H2O2。

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