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首页> 外文期刊>Electroanalysis >Electrochemical and Spectroscopic Studies on the Interaction of Gatifloxacin, Moxifloxacin and Sparfloxacin with DNA and Their Analytical Applications
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Electrochemical and Spectroscopic Studies on the Interaction of Gatifloxacin, Moxifloxacin and Sparfloxacin with DNA and Their Analytical Applications

机译：加替沙星，莫西沙星和司帕沙星与DNA相互作用的电化学和光谱研究及其分析应用

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摘要

The electrochemical oxidation of the three fluoroquinolone drugs FQs: gatifloxacin GTF, moxifloxacin MXF and sparfloxacin SPF, at the bare and DNA-modified glassy carbon electrodes has been studied by voltammetric techniques. The three FQs showed one irreversible oxidation peak at potential range 0.85-0.91 V vs. Ag-AgCl, in phosphate buffer of pH 7.0. Differential pulse voltammetry (DPV) and UV-absorption spectroscopic techniques were employed to probe the interaction between the FQs and calf thymus double stranded deoxyribonucleic acid (ds CT-DNA). From electrochemical data, the binding constant between DNA and the gatifloxacin, moxifloxacin and sparfloxacin are calculated to be 3228, 2596 and 2857 M~ (-1)respectively. Based on electrochemical and spectroscopic results, the mode of binding of fluoroquinolone to DNA through combined effect of intercalation and electrostatic interaction was concluded. A detection scheme based on a preconcentration and differential pulse voltammetric (DPV) determination at dsDNA modified glassy carbon electrode (DNA/GCE) was proposed for the trace determination of the studied analytes. The developed method was successfully applied to the determination of the FQs in pharmaceutical formulations.
机译：已经通过伏安法研究了三种氟喹诺酮药物FQs的电化学氧化：加替沙星GTF，莫西沙星MXF和司巴沙星SPF在裸露的和DNA修饰的玻璃碳电极上的电化学氧化。在pH 7.0的磷酸盐缓冲液中，三个FQ在相对于Ag-AgCl的0.85-0.91 V电位范围内显示出一个不可逆的氧化峰。差分脉冲伏安法（DPV）和紫外吸收光谱技术被用来探测FQs和小牛胸腺双链脱氧核糖核酸（ds CT-DNA）之间的相互作用。根据电化学数据，DNA与加替沙星，莫西沙星和司巴沙星之间的结合常数分别计算为3228、2596和2857 M〜（-1）。根据电化学和光谱结果，总结了通过嵌入和静电相互作用共同作用的氟喹诺酮与DNA的结合方式。提出了在dsDNA修饰的玻碳电极（DNA / GCE）上基于预浓缩和差分脉冲伏安法（DPV）测定的检测方案，用于痕量分析物的测定。所开发的方法已成功地应用于药物制剂中FQ的测定。

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