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首页> 外文期刊>Electroanalysis >A High Performance Electrochemical Biosensing Platform for Glucose Detection and IgE Aptasensing Based on Fe_3O_4/Reduced Graphene Oxide Nanocomposite
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A High Performance Electrochemical Biosensing Platform for Glucose Detection and IgE Aptasensing Based on Fe_3O_4/Reduced Graphene Oxide Nanocomposite

机译:基于Fe_3O_4 /还原型氧化石墨烯纳米复合材料的葡萄糖电化学检测和IgE传感的高效电化学生物传感平台

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摘要

A Fe_3O_4 magnetic nanoparticles/reduced graphene oxide nanocomposite modified glassy carbon electrode (Fe_3O_4/r-GO/GCE) was further expanded into the design of a high performance electrochemical biosensing platform. First, direct electrochemistry of glucose oxidase(GOx) immobilized onto Fe_3O_4/r-GO/GCE and its biocatalytic activity toward glucose oxidation was investigated. Then, differential pulse voltammetry (DPV) was used to investigate the electrochemical oxidation behavior of four DNA free bases of guanine (G), adenine (A), thymine (T) and Cytosine (C) at Fe_3O_4/r-GO/GCE. In addition, a label-free electrochemical impedance spectroscopy (EIS) method of detection was developed for Immunoglobulin E (IgE) in which the binding of IgE-specific aptamers immobilized on Fe_3O_4/r-GO/GCE leads to impedance changes associated with IgE binding events.
机译:Fe_3O_4磁性纳米粒子/还原氧化石墨烯纳米复合改性玻璃碳电极(Fe_3O_4 / r-GO / GCE)进一步扩展到高性能电化学生物传感平台的设计中。首先,研究了固定在Fe_3O_4 / r-GO / GCE上的葡萄糖氧化酶(GOx)的直接电化学及其对葡萄糖氧化的生物催化活性。然后,使用差分脉冲伏安法(DPV)研究了鸟嘌呤(G),腺嘌呤(A),胸腺嘧啶(T)和胞嘧啶(C)的四个DNA游离碱基在Fe_3O_4 / r-GO / GCE上的电化学氧化行为。此外,针对免疫球蛋白E(IgE)开发了一种无标记电化学阻抗谱(EIS)检测方法,其中固定在Fe_3O_4 / r-GO / GCE上的IgE特异性适体的结合导致与IgE结合相关的阻抗变化事件。

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