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Multiwalled Carbon Nanotubes Modified by NTA-Copper Complex for Label-Free Electrochemical Immunosensor Detection

机译:NTA-铜配合物修饰的多壁碳纳米管用于无标记电化学免疫传感器检测

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摘要

A biomaterial based on multiwalled carbon nanotubes (MWCNTs) modified with His-tagged single chain D-dimer antibody trough nitrilo-triacetic-acid (NTA)/copper complex was designed for monitoring D-dimers, which were used as biomarkers of Deep Vein Thrombosis (DVT). The immunosensing evaluation of D-Dimer was followed by measuring the redox process of such complex. We demonstrate that using simple chemistry and exploiting the high surface area, electronic properties of MWCNTs and redox signal of the NTA/Cu~(2+) complex an efficient electrochemical immunosensor for the evaluation of wide range of D-dimer concentrations was obtained.
机译:设计了一种基于多壁碳纳米管(MWCNT)的生物材料,该材料经His标记的单链D-二聚体抗体槽亚硝基三乙酸(NTA)/铜复合物修饰,用于监测D-二聚体,用作深静脉血栓形成的生物标志物(DVT)。通过测量这种复合物的氧化还原过程来进行D-二聚体的免疫传感评估。我们证明,使用简单的化学方法并利用高表面积,MWCNTs的电子特性和NTA / Cu〜(2+)络合物的氧化还原信号,可获得一种有效的电化学免疫传感器,用于评估各种D-二聚体浓度。

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