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Comparative Analysis of Cellular Prion Detection by Mass-Sensitive Immunosensors

机译:质敏免疫传感器检测细胞Pri病毒的比较分析

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We performed a comparative study of the detection of human cellular prions (PrP~C) with a quartz crystal microbalance (QCM) immunosensor using various antibodies against PrP~C. The sensing layer was formed by immobilized antibodies on a surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) with attached protein A. The sensitivity of detection depended on the kind of antibody used and the best detection limit was 0.8 nM. PrPC detection in 10 times diluted blood plasma revealed more complicated behavior of motional resistance, which is evidence on viscosity contribution. The sensor recovery was 82.2%, suggesting certain matrix effects on prion detection.
机译:我们对石英细胞微天平(QCM)免疫传感器使用各种抗PrP〜C的抗体检测人细胞的病毒(PrP〜C)进行了比较研究。传感层是通过将抗体固定在带有附着蛋白A的第四代(G4)聚酰胺基胺树枝状聚合物(PAMAM)的表面上形成的。检测的灵敏度取决于所用抗体的种类,最佳检测限为0.8 nM。在稀释的10倍血浆中进行PrPC检测可发现运动阻力的行为更为复杂,这是粘度贡献的证据。传感器的回收率为82.2%,表明某些基质对病毒的检测有影响。

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