Detection of listeria monocytogenes by electrochemical impedance spectroscopy

Radhakrishnan R.; Jahne M.; Rogers S.; Suni I.I.

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Detection of listeria monocytogenes by electrochemical impedance spectroscopy

机译：电化学阻抗谱法检测单核细胞增生李斯特菌

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Listeria monocytogenes is detected by electrochemical impedance spectroscopy using a mouse monoclonal antibody immobilized onto an Au electrode. This yields sensitivities of 0.825kΩ cm2/(CFU/mL) and 1.129kΩ cm~2/(CFU/mL) and detection limits of 5CFU/mL and 4CFU/mL for ideal solutions and filtered tomato extract, respectively. Control experiments with an Au electrode onto which a mouse monoclonal antibody to GAPDH is immobilized demonstrate that non-specific adsorption is insignificant for the system and methodology studied here. Control experiments with Salmonella enterica demonstrate no cross-reactivity to this food pathogen. Potential technological hurdles to development of a multiplexed impedance biosensor for food pathogens are also discussed.

机译：使用固定在Au电极上的小鼠单克隆抗体，通过电化学阻抗光谱法检测单核细胞增生李斯特菌。理想溶液和过滤后的番茄提取物的灵敏度分别为0.825kΩcm2 /（CFU / mL）和1.129kΩcm〜2 /（CFU / mL），检出限分别为5CFU / mL和4CFU / mL。在其上固定了针对GAPDH的小鼠单克隆抗体的Au电极的对照实验表明，非特异性吸附对于此处研究的系统和方法而言无关紧要。肠沙门氏菌的对照实验表明，与该食物病原体无交叉反应。还讨论了开发针对食品病原体的多路阻抗生物传感器的潜在技术障碍。

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《Electroanalysis》 |2013年第9期|共7页
作者
Radhakrishnan R.; Jahne M.; Rogers S.; Suni I.I.;
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原文格式 PDF
正文语种 eng
中图分类 54.24;
关键词
Biosensors; Electrochemical Impedance Spectroscopy; Food Pathogens; Immunoassays;

机译：生物传感器;电化学阻抗谱;食品病原体;免疫测定;

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1. Detection of listeria monocytogenes by electrochemical impedance spectroscopy [J] . Radhakrishnan R., Jahne M., Rogers S., Electroanalysis . 2013,第9期

机译：电化学阻抗谱法检测单核细胞增生李斯特菌
2. A sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of Listeria monocytogenes using an immobilization-free interdigitated array microelectrode [J] . Chen Qi, Lin Jianhan, Gan Chengqi, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2015,第Null期

机译：基于免疫磁分离和脲酶催化的灵敏阻抗生物传感器，可使用无固定叉指阵列微电极快速检测单核细胞增生李斯特菌
3. Efficient Separation and Sensitive Detection of Listeria monocytogenes Using an Impedance Immunosensor Based on Magnetic Nanoparticles, a Microfluidic Chip, and an Interdigitated Microelectrode [J] . DAMIRA A. KANAYEVA, RONGHUI WANG, DOUGLAS RHOADS, Journal of food protection . 2012,第11期

机译：使用基于磁性纳米颗粒，微流控芯片和指状微电极的阻抗免疫传感器高效分离和敏感检测单核细胞增生李斯特菌
4. Impedance spectroscopy and biochip sensor for detection of Listeria monocytogenes [C] . Arun K.Bhunia, Ziad W.Jaradat, Kristen Naschansky, Conference on photonic detection and intervention technologies for safe food . 2001

机译：阻抗光谱和Biochip传感器，用于检测李斯特菌单核细胞增生
5. Efficient separation and sensitive detection of Listeria monocytogenes using magnetic nanoparticles, microfluidics and interdigitated microelectrode based impedance immunosensor. [D] . Kanayeva, Damira Ashimkhanovna. 2010

机译：使用磁性纳米颗粒，微流控技术和基于指状微电极的阻抗免疫传感器对单核细胞增生李斯特菌进行高效分离和灵敏检测。
6. Flow-Through Electrochemical Biosensor for the Detection of Listeria monocytogenes Using Oligonucleotides [O] . Cheryl M. Armstrong, Joe Lee, Andrew G. Gehring, 2021

机译：使用寡核苷酸检测李斯特菌单核细胞增生的流通电化学生物传感器
7. Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes [O] . Maria Chiriacò, Ilaria Parlangeli, Fausto Sirsi, 2018

机译：用于检测食品病原体李斯特菌单核细胞增生的阻抗感应平台

Detection of listeria monocytogenes by electrochemical impedance spectroscopy

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