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首页> 外文期刊>Electroanalysis >Development and application of oxysilane sol-gel electrochemical glucose biosensors based on cobalt hexacyanoferrate modified carbon film electrodes
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Development and application of oxysilane sol-gel electrochemical glucose biosensors based on cobalt hexacyanoferrate modified carbon film electrodes

机译：六氰合铁酸钴修饰碳膜电极的氧硅烷溶胶-凝胶电化学葡萄糖生物传感器的开发与应用

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摘要

The evaluation and characterization of a new glucose oxidase (GOx) electrochemical enzyme biosensors on a carbon film electrode support and using two types of sol-gel precursor mixture for enzyme immobilization was carried out in order to be employed as a short-time-use or as a disposable sensor. Carbon film electrodes were modified with films of CoHCF mediator by potential cycling. The sol-gel was prepared using a combination of 3-glycidoxypropyltrimethoxysilane (GOPMOS) with methyltrimethoxysilane (MTMOS) or tetraethoxysilane (TEOS). The best biosensor sensitivity (ca. 139 nA mM(-1)) and limit of detection (24 mu M) to glucose was obtained when a combination of GOPMOS with MTMOS sol - gel was used, but for a short linear range (0 - 0.2 mM) and using a big concentration of immobilized enzyme (ca. 3.5%). The longest linear range (0-1 mM) was obtained for the biosensor prepared with GOPMOS and TEOS sol-gel but with lower sensitivity (19.7 nA mM(-1)) and a bigger detection limit (71 mu M) with a concentration of immobilized enzyme of 1%. The biosensors were used at 0.0 V vs. SCE with minimal interference from carboxylic acids and phenol; only ascorbate showed a significant interference but this effect was decreased to 3% by coating with Nafion polymer. The biosensor was successfully applied to the analysis of glucose in wine samples.
机译：为了在短时间内使用或使用两种类型的溶胶-凝胶前体混合物固定酶，对碳膜电极载体上的新型葡萄糖氧化酶（GOx）电化学酶生物传感器进行了评估和表征。作为一次性传感器。碳膜电极通过CoHCF介体膜通过电位循环进行修饰。使用3-环氧丙氧基丙基三甲氧基硅烷（GOPMOS）与甲基三甲氧基硅烷（MTMOS）或四乙氧基硅烷（TEOS）的组合制备溶胶-凝胶。当结合使用GOPMOS和MTMOS溶胶-凝胶时，可获得最佳的生物传感器灵敏度（约139 nA mM（-1））和对葡萄糖的检测极限（24μM），但线性范围较短（0- 0.2 mM）并使用高浓度的固定化酶（约3.5％）。用GOPMOS和TEOS溶胶-凝胶制备的生物传感器获得了最长的线性范围（0-1 mM），但灵敏度较低（19.7 nA mM（-1）），检测限较大（71μM），浓度为固定化酶为1％。生物传感器在0.0 V vs. SCE的条件下使用，对羧酸和苯酚的干扰最小。仅抗坏血酸盐显示出显着的干扰，但通过用Nafion聚合物涂层将其影响降低至3％。该生物传感器已成功应用于葡萄酒样品中葡萄糖的分析。

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