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首页> 外文期刊>Electroanalysis >Single-Walled Carbon Nanotubes Modified Graphite Electrodes for Electrochemical Monitoring of Nucleic Acids and Biomolecular Interactions
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Single-Walled Carbon Nanotubes Modified Graphite Electrodes for Electrochemical Monitoring of Nucleic Acids and Biomolecular Interactions

机译：单壁碳纳米管修饰石墨电极用于核酸和生物分子相互作用的电化学监测。

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摘要

Single-walled carbon nanotubes (SWCNT) modified disposable graphite electrodes (SWCNT-PGEs) were investigated in our study for the improved electrochemical monitoring of nucleic acids and biomolecular interactions based on the higher signal enhancement comparison to bare PGEs. The surface morphologies of bare PGE and SWCNT-PGE were firstly explored using scanning electron microscopy (SEM) analysis. The easy surface modification of disposable graphite electrodes with carbon nanotubes was performed by passive adsorption, and DNA was then immobilized onto the SWCNT-PGEs by the formation of covalent coupling between the carboxylated ends of nanotubes and the amine group in the guanine bases of DNA. The overall performance of SWCNT-PGEs has also been studied, and discussed in terms of optimum analytical conditions; such as, the effect of pretreatment step, CNT concentration, DNA concentration, etc. The reproducible detection of DNA represent a very attractive approach for the further detection of interaction between the anticancer drug, daunorubicin (DNR) and double stranded DNA (dsDNA). Voltammetric results were complemented with electrochemical impedance spectroscopy (EIS), that was used to characterize the successful construction of carbon nanotubes modification onto the surface of PGEs.
机译：在我们的研究中，对单壁碳纳米管（SWCNT）改性的一次性石墨电极（SWCNT-PGEs）进行了研究，以基于与裸露的PGE相比更高的信号增强，改进了对核酸和生物分子相互作用的电化学监测。首先利用扫描电子显微镜（SEM）分析了裸露的PGE和SWCNT-PGE的表面形态。用碳纳米管对一次性石墨电极进行简单的表面修饰是通过被动吸附进行的，然后通过在纳米管的羧基末端与鸟嘌呤碱基中的胺基之间形成共价偶联，将DNA固定在SWCNT-PGE上。还对SWCNT-PGE的整体性能进行了研究，并根据最佳分析条件进行了讨论。例如，预处理步骤的效果，CNT浓度，DNA浓度等。DNA的可重复检测是进一步检测抗癌药物柔红霉素（DNR）与双链DNA（dsDNA）之间相互作用的非常有吸引力的方法。伏安法结果与电化学阻抗谱（EIS）相辅相成，用于表征碳纳米管修饰在PGE表面上的成功构建。

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