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首页> 外文期刊>Electroanalysis >The Dependence of the Sensitivity and Reliability of Contactless Conductivity Detection on the Wall Thickness of Electrophoretic Fused-Silica Capillaries
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The Dependence of the Sensitivity and Reliability of Contactless Conductivity Detection on the Wall Thickness of Electrophoretic Fused-Silica Capillaries

机译：非接触式电导率检测的灵敏度和可靠性对电泳熔融石英毛细管壁厚的依赖性

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摘要

A contactless conductivity detector (CD) performance has been tested on a simple capillary electrophoretic separation in a standard fused-silica capillary with an external diameter of 360 mu m and in a thin-walled capillary (an external diameter of 150 mu m); the internal diameters of the two capillaries were identical, equal to 75 mu m. Potassium and sodium ions have been separated in a morpholinoethanesulfonic acid/histidine background electrolyte (MES/His), over a wide range of its concentrations (0 - 100 mM). At low MES/His concentrations, the CD response, obtained from the height of the potassium peak, is by 100 to 200 per cent higher for the thin-walled capillary and the calibration dependences are linear, in contrast to the thick-walled capillary. These differences between the two capillaries decrease with increasing MES/His concentration, the CD response in the thin-walled capillary is then higher by mere 20 per cent and the calibration dependences are linear in both the capillaries. The highest sensitivities have been obtained at a MES/His concentration of 50 mM, with LOD values for potassium ion of 2.0 and 2.6 mu M, in the thin- and thick-walled capillaries, respectively. The signal-to-noise ratios and the plate counts are generally similar for the two capillaries. It follows from the results that special thin-walled capillaries can be advantageous when background electrolytes with very low conductivities must be employed.
机译：非接触式电导检测器（CD）的性能已经在标准的熔融石英毛细管（外径为360μm）和薄壁毛细管（外径为150μm）中进行了简单的毛细管电泳分离;两个毛细管的内径相同，等于75微米。钾和钠离子已在吗啉代乙磺酸/组氨酸背景电解质（MES / His）中分离，浓度范围很广（0-100 mM）。在低MES / His浓度下，与厚壁毛细管相比，薄壁毛细管从钾峰高度获得的CD响应要高100％至200％，并且校准依赖性是线性的。两个毛细管之间的差异随MES / His浓度的增加而减小，然后薄壁毛细管中的CD响应仅高出20％，并且两个毛细管中的校准依赖性均呈线性关系。在薄壁和厚壁毛细管中，在MES / His浓度为50 mM时，钾离子的LOD值为2.0和2.6μM时，灵敏度最高。这两个毛细管的信噪比和板数通常相似。从结果可以得出，当必须使用电导率非常低的本底电解质时，特殊的薄壁毛细管可能是有利的。

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