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首页> 外文期刊>Electroanalysis >Recording the Reaction Process of Loop-Mediated Isothermal Amplification (LAMP) by Monitoring the Voltammetric Response of 2′-Deoxyguanosine 5′-Triphosphate
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Recording the Reaction Process of Loop-Mediated Isothermal Amplification (LAMP) by Monitoring the Voltammetric Response of 2′-Deoxyguanosine 5′-Triphosphate

机译：通过监测2'-脱氧鸟苷5'-三磷酸的伏安法反应记录回路介导的等温扩增（LAMP）的反应过程

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摘要

We describe here a novel strategy for recording the reaction process of loop-mediated isothermal amplification (LAMP) by monitoring the voltammetric response of 2′-deoxyguanosine 5′-triphosphate (dGTP). Unlike the other three kinds of reactive substrates for DNA synthesis in LAMP reaction, dGTP exhibits sensitive voltammetric response at the carbon nanotube array electrode. When the LAMP reaction occurs, the concentration of dGTP decreases accordingly, bringing forth the decrease of the anodic peak current (i_(pa)). In inversion, the decrease of the i_(pa) of dGTP was used to characterize the reaction process of LAMP. The relationships among the LAMP reaction time, the initial quantity of template DNA and the value change of the i_(pa) were studied. The results indicate that the protocol integrated LAMP and voltammetric techniques can be used for not only qualitative gene discrimination but also quantitative gene assay in a wide range. The malB gene extracted from common strains of Escherichia coli cells was tested as a model. The detecting results of LAMPs obtained by voltammetric method were in good agreement with those by optical-based methods (gel electrophoresis and fluorescent dye).
机译：我们在这里描述了一种通过监测2'-脱氧鸟苷5'-三磷酸（dGTP）的伏安响应来记录环介导的等温扩增（LAMP）反应过程的新策略。与LAMP反应中用于DNA合成的其他三种反应性底物不同，dGTP在碳纳米管阵列电极上显示出灵敏的伏安响应。当发生LAMP反应时，dGTP的浓度相应降低，导致阳极峰值电流（i_（pa））降低。反过来，dGTP的i_（pa）的减少被用来表征LAMP的反应过程。研究了LAMP反应时间，模板DNA初始量和i_（pa）值变化之间的关系。结果表明，该协议集成了LAMP和伏安技术，不仅可以用于定性基因鉴别，而且还可以广泛地用于定量基因测定。从大肠杆菌细胞的普通菌株中提取的malB基因作为模型进行了测试。通过伏安法获得的LAMPs的检测结果与基于光学的方法（凝胶电泳和荧光染料）的检测结果吻合良好。

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