• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Endothelium: Journal of endothelial cell research >Metabolism of dynorphins by peptidases of pulmonary artery endothelial cells.
【24h】

Metabolism of dynorphins by peptidases of pulmonary artery endothelial cells.

机译：强啡肽通过肺动脉内皮细胞肽酶的代谢。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Degradation of several dynorphins by peptidases expressed in cultured porcine pulmonary artery endothelial cells was studied by incubation of the peptide in cell suspensions followed by electrospray ionization and tandem mass spectrometric analyses. Under the in vitro conditions applied, only the metabolism of dynorphin A1-8 occurred in a significant extent. Studies involving specific peptidase inhibitors indicated that mainly bestatin-sensitive aminopeptidases, thiorphan-sensitive endopeptidases, and cFPAAF-pAB-sensitive endopeptidases expressed by the endothelial cells were involved in the process that converted dynorphin A1-8 to dynorphin A2-8, dynorphin A1-6, and leucine enkephalin (dynorphin A1-5), respectively. These peptidases may form a metabolic barrier for the cellular penetration of intact dynorphin A1-8 and/or control effects of the circulating peptide on endothelial opioid receptors of the cells.
机译：通过在细胞悬液中孵育肽，然后进行电喷雾电离和串联质谱分析，研究了培养的猪肺动脉内皮细胞中表达的肽酶对几种强啡肽的降解作用。在所应用的体外条件下，仅强啡肽A1-8的代谢显着发生。涉及特定肽酶抑制剂的研究表明，内皮细胞表达的主要是Bestatin敏感的氨基肽酶，thiorphan敏感的内肽酶和cFPAAF-pAB敏感的内肽酶参与了将强啡肽A1-8转化为强啡肽A2-8，强啡肽A1-的过程6和亮氨酸脑啡肽（强啡肽A1-5）。这些肽酶可能形成完整的强啡肽A1-8的细胞渗透和/或循环肽对细胞内皮阿片受体的控制作用的代谢屏障。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号