• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Endothelium: Journal of endothelial cell research >New human microvascular endothelial cell lines with specific adhesion molecules phenotypes.
【24h】

New human microvascular endothelial cell lines with specific adhesion molecules phenotypes.

机译：具有特定粘附分子表型的新型人微血管内皮细胞系。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vascular endothelial cells recognize blood-borne circulating cells and allow them to extravasate in a tissue-specific manner. Because this property determines the selectivity of lymphocyte homing, it is fundamental in physiological as well as pathological processes (inflammation, autoimmune diseases, metastasis). As a tool to assess the molecular basis of endothelium selectivity, microvascular endothelial cell lines of distinct tissue origin were established. Endothelial cells, isolated from lymphoid tissues (lymph nodes and appendix) and from nonlymphoid immune sites--intestine, lung, and skin--were immortalized in vitro. Their general endothelial characteristics, such as the presence of von Willebrand factor (wWf), angiotensin-converting enzyme (ACE), VE-cadherin, and the intracellular E-selectin, were preserved. This article shows that these cell lines display phenotypic characteristics related to their tissue origin. Hence, endothelial cells from lymph nodes expressed peripheral lymph node addressins (PNAds). Endothelial cells from nonlymphoid tissues were ICAM-1 (intercellular adhesion molecule-1) and CD49e positive, whereas P-selectin was not equally distributed among the cell lines. Endothelial cells from mucosal sites reacted with antibody against human MAdCAM-1 (mucosal addressin cell adhesion molecule). In the adhesion test, lymphoid and myeloid cells adhere to endothelial cell lines in a distinct manner. These lines could be useful to study molecular mechanisms involved in tissue-specific cell-cell interaction.
机译：血管内皮细胞识别血液传播的循环细胞，并使其以组织特异性方式外渗。因为此特性决定了淋巴细胞归巢的选择性，所以它在生理和病理过程（炎症，自身免疫性疾病，转移）中都至关重要。作为评估内皮选择性分子基础的工具，建立了具有不同组织起源的微血管内皮细胞系。从淋巴组织（淋巴结和阑尾）和非淋巴免疫部位（肠，肺和皮肤）分离出的内皮细胞在体外永生。保留了它们的一般内皮特征，如存在血管性血友病因子（wWf），血管紧张素转化酶（ACE），VE-钙粘着蛋白和细胞内E-选择素。本文显示这些细胞系显示与其组织起源有关的表型特征。因此，来自淋巴结的内皮细胞表达外周淋巴结地址蛋白（PNAds）。非淋巴组织的内皮细胞为ICAM-1（细胞间粘附分子-1）和CD49e阳性，而P-选择蛋白在细胞系中分布不均。来自粘膜部位的内皮细胞与针对人MAdCAM-1的抗体（粘膜地址蛋白细胞粘附分子）发生反应。在粘附试验中，淋巴样和髓样细胞以独特的方式粘附于内皮细胞系。这些系可能有助于研究组织特异性细胞间相互作用所涉及的分子机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号