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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Validation of endothelial progenitor cells in human umbilical veins and the isolated endothelial cells.
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Validation of endothelial progenitor cells in human umbilical veins and the isolated endothelial cells.

机译：验证人脐静脉内皮祖细胞和分离的内皮细胞。

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摘要

To detect endothelial progenitor cells in human umbilical veins and isolated endothelial cells, the authors examined protein and mRNA expression levels of cell surface markers for endothelial progenitor cells in human umbilical veins before and after trypsin treatment and at different passages of the isolated endothelial cells. CD133(+) (2.14 +/- 0.57 per mm) and KDR(+) (35.74 +/- 8.28 per mm) cells were observed in the intima of umbilical veins. The amounts of CD133(+), KDR(+), CD34(+), and CD105(+) cells decreased in the intima after trypsin treatment, whereas the percent of CD133(+) and KDR(+)cells in the media did not change significantly. Moreover, similar protein and mRNA expression levels of CD133 and KDR were detected in the umbilical veins before and after trypsin treatment. In the isolated cells from umbilical veins, the percent of CD133(+) and CD34(+) cells in P1 was 3.43% +/- 3.85%, which was higher than those in P3 (0.17% +/- 0.21%, p = 0.005) and P6 (0.14% +/- 0.18%, p = .001). The mRNA expression levels of CD133 and CD105 were down-regulated in later passages compared to those in P1, whereas the expression level of KDR was up-regulated in late passages. Thus it is suggested that endothelial progenitor cells reside in the distinct zone (e.g., initma and media) of human umbilical veins, and retain the capacity of differentiation to endothelial cells in vitro.
机译：为了检测人脐静脉和分离的内皮细胞中的内皮祖细胞，作者检查了胰蛋白酶处理前后，分离的内皮细胞不同传代时人脐静脉中的内皮祖细胞的细胞表面标志物的蛋白质和mRNA表达水平。在脐静脉内膜中观察到CD133（+）（2.14 +/- 0.57 / mm）和KDR（+）（35.74 +/- 8.28 / mm）细胞。胰蛋白酶处理后内膜中CD133（+），KDR（+），CD34（+）和CD105（+）细胞的数量减少，而培养基中CD133（+）和KDR（+）细胞的百分比却降低了变化不大。此外，在胰蛋白酶治疗之前和之后的脐静脉中检测到相似的蛋白质和mRNA表达水平的CD133和KDR。在从脐静脉分离的细胞中，P1中CD133（+）和CD34（+）细胞的百分比为3.43％+/- 3.85％，高于P3中的百分比（0.17％+/- 0.21％，p = 0.005）和P6（0.14％+/- 0.18％，p = 0.001）。与P1相比，CD133和CD105的mRNA表达水平在后期传代中被下调，而KDR的表达水平在后期传代中被上调。因此，提示内皮祖细胞位于人脐静脉的不同区域（例如，初始细胞和中膜），并且在体外保留向内皮细胞分化的能力。

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