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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Isolation and characterization of human bone-derived endothelial cells.
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Isolation and characterization of human bone-derived endothelial cells.

机译：人骨来源的内皮细胞的分离和表征。

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摘要

Historically, the etiology of local bone pathologies, such as avascular necrosis, has been related to intravascular occlusion. Recent reports have highlighted the occlusion of arteries, venules, and/or capillaries in bone tissue. Endothelium of bone presumably participates locally in the formation of the microvascular thrombosis. It is also known that endothelial cells (ECs) play a central role in angiogenesis, a process seen in osteosarcoma, amongst other bone diseases. Given the well-recognized heterogeneity of ECs throughout the body, investigations of local bone disease related to endothelium processes may be more appropriately targeted on bone ECs rather than other primary ECs or an immortalized EC line. In the current study, mechanical and enzymatic methods are described to isolate ECs from cancellous human bone tissue followed by immunomagnetic bead separation to purify the cell populations. The human bone-derived endothelial cells (hBDECs) were characterized based on endothelial cell antigen expression and functional assays. This study is the first report of isolation and expansion of ECs from human bone tissue. Isolation of hBDECs in human vascular bone diseases may facilitate the study of the molecular and/or genetic abnormalities in the vasculature system that contributes to the initiation and/or progression of the disease.
机译：历史上，局部骨骼病理的病因，例如无血管坏死，与血管内阻塞有关。最近的报道突出了骨组织中动脉，小静脉和/或毛细血管的阻塞。骨的内皮大概参与了微血管血栓形成的形成。还已知内皮细胞（EC）在血管生成中起着核心作用，血管生成是骨肉瘤以及其他骨骼疾病中常见的过程。考虑到整个人体EC的异质性，与内皮过程相关的局部骨疾病的研究可能更适合针对骨EC，而不是其他主要EC或永生化EC系列。在当前研究中，描述了机械和酶促方法从松质人骨组织中分离EC，然后进行免疫磁珠分离以纯化细胞群体。基于内皮细胞抗原表达和功能测定来表征人骨源性内皮细胞（hBDEC）。这项研究是从人骨组织中分离和扩增EC的第一份报告。在人类血管性骨疾病中分离hBDEC可能有助于研究有助于疾病发作和/或进展的脉管系统中的分子和/或遗传异常。

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