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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Signal transduction and procoagulant state of human cord blood--progenitor-derived endothelial cells after interleukin-1alpha stimulation.
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Signal transduction and procoagulant state of human cord blood--progenitor-derived endothelial cells after interleukin-1alpha stimulation.

机译：IL-1α刺激后人脐带血祖细胞内皮细胞的信号转导和促凝状态。

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Isolation of endothelial progenitors from human umbilical cord blood generated great hope in vascular tissue engineering. However, before clinical use, progenitor derived endothelial cells (PDECs) have to be compared with mature endothelial cells (ECs). The aim of this study was to explore the behavior of PDECs exposed to a proinflammatory cytokine (interleukin-1alpha; IL-1alpha) according to the mitogen-activated protein (MAP) kinase and nuclear factor (NF)-kappaB signal transduction pathways as well as procoagulant activity (PCA). CD34(+) mononuclear cells were isolated using magnetic beads, cultured, and compared with human saphenous vein ECs (HSVECs). PDECs express endothelial markers: CD31, VE-cadherin, von Willebrand factor, KDR, and incorporate acetylated low-density lipoprotein (Dil-Ac-LDL). IL-1alpha similarly activates c-Jun N-terminal protein kinase (JNK) and p38 pathways in HSVECs and PDECs, whereas extracellular signal-related kinase (ERK)1/2 phosphorylation is lower in PDECs than in HSVECs. Low ERK1/2 phosphorylation in PDECs was specific to IL-1alpha as vascular endothelial growth factor (VEGF) similarly stimulated ERK1/2 pathway. With respect to inhibitor of NF-kappa B (Ikappa B) degradation, NF-kappa B translocation and phosphorylation, the NF-kappa B pathway is comparable in HSVECs and PDECs after stimulation. PCA and tissue factor level induced by IL-1alpha are lower in PDECs than in HSVECs. Thus, our data show that PDECs display the characteristics of functional mature ECs under IL-1alpha stimulation. However, we observed significant differences between PDECs and HSVECs related to both ERK1/2 pathway activation and tissue factor production.
机译：从人脐带血中分离内皮祖细胞在血管组织工程中产生了巨大希望。但是，在临床使用之前，必须将祖细胞衍生的内皮细胞（PDEC）与成熟的内皮细胞（EC）进行比较。这项研究的目的是根据促分裂原活化蛋白（MAP）激酶和核因子（NF）-kappaB信号转导途径探讨暴露于促炎细胞因子（白介素-1α; IL-1alpha）的PDEC的行为作为促凝血活性（PCA）。使用磁珠分离CD34（+）单核细胞，进行培养，并与人隐静脉EC（HSVEC）进行比较。 PDECs表达内皮标记物：CD31，VE-钙黏着蛋白，von Willebrand因子，KDR，并掺入乙酰化的低密度脂蛋白（Dil-Ac-LDL）。 IL-1alpha同样激活HSVEC和PDEC中的c-Jun N末端蛋白激酶（JNK）和p38途径，而PDEC中的细胞外信号相关激酶（ERK）1/2磷酸化低于HSVEC。 PDEC中的低ERK1 / 2磷酸化特异于IL-1alpha，因为血管内皮生长因子（VEGF）类似地刺激了ERK1 / 2途径。关于NF-κB（Ikappa B）降解，NF-κB易位和磷酸化的抑制剂，在刺激后，HSVEC和PDEC中的NF-κB途径相当。 IL-1α诱导的PCA和组织因子水平在PDEC中低于在HSVEC中。因此，我们的数据表明PDECs在IL-1alpha刺激下显示出功能成熟的ECs的特征。但是，我们观察到PDEC和HSVEC之间的显着差异与ERK1 / 2途径激活和组织因子产生有关。

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