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首页> 外文期刊>Endothelium: Journal of endothelial cell research >Isolation, culture and characterization of endothelial cells from human hypertrophic scar.
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Isolation, culture and characterization of endothelial cells from human hypertrophic scar.

机译：人肥厚性瘢痕中内皮细胞的分离，培养和鉴定。

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摘要

Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in the pathogenesis of scar development. Recently, the authors developed a reliable technique to obtain endothelial cells from hypertrophic scars that involved separation of cells from the scar tissue matrix and isolation from other cell types. Using phase-contract and electron microscopy, the cells were observed to have a characteristic morphology consistent with cells of endothelial origin. The cells were further characterized as endothelial cells by assessment of endothelin (ET)-1 and intercellular adhesion molecule (ICAM) mRNA expression, and the presence of factor VIII antigen, CD34, CD31, and VE-cadherin. This isolation method provides a simple method for culturing endothelial cells from hypertrophic scar tissue and should prove useful for studying the role of endothelial cell involvement in scar formation.
机译：尽管尚不清楚瘢痕形成的病因，但先前的研究表明内皮细胞在瘢痕形成的发病机理中起作用。最近，作者开发了一种可靠的技术，可以从肥厚性瘢痕中获得内皮细胞，该技术涉及从瘢痕组织基质中分离细胞并与其他细胞类型分离。使用相合同和电子显微镜，观察到细胞具有与内皮来源的细胞一致的特征形态。通过评估内皮素（ET）-1和细胞间粘附分子（ICAM）mRNA表达，以及因子VIII抗原，CD34，CD31和VE-钙粘着蛋白的存在，进一步将细胞表征为内皮细胞。这种分离方法提供了一种从肥厚性瘢痕组织中培养内皮细胞的简单方法，应该被证明对研究内皮细胞参与瘢痕形成的作用是有用的。

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