Thrombin downregulates thrombomodulin expression and activity in primary human endothelial cells.

Seguin C; Abid MR; Spokes KC; Aird WC

首页> 外文期刊>Endothelium: Journal of endothelial cell research >Thrombin downregulates thrombomodulin expression and activity in primary human endothelial cells.

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Thrombin downregulates thrombomodulin expression and activity in primary human endothelial cells.

机译：凝血酶下调人类原代内皮细胞中的血栓调节蛋白表达和活性。

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Thrombomodulin (TM) is a cell surface anticoagulant glycoprotein that plays a key role in the protein C pathway. TM expression in endothelial cells may be modulated by a variety of extracellular signals. Most notably, TM has been shown to be downregulated by inflammatory mediators, such as tumor necrosis factor-alpha and lipopolysaccharide. The objective of this study was to determine the effect of thrombin on TM expression and activity. Thrombin resulted in reduced TM in primary cultures of human endothelial cells by approximately 40% at the level of mRNA, protein, and activity. These effects were blocked by the thrombin inhibitor hirudin. These results suggest that activation of the coagulation cascade may result in a positive-feedback loop consisting of thrombin-mediated repression of TM-dependent protein C activation.

机译：血栓调节蛋白（TM）是一种细胞表面抗凝糖蛋白，在C蛋白途径中起关键作用。内皮细胞中的TM表达可以通过多种细胞外信号来调节。最值得注意的是，TM已被炎症介质（例如肿瘤坏死因子-α和脂多糖）下调。这项研究的目的是确定凝血酶对TM表达和活性的影响。凝血酶导致人内皮细胞原代培养物中TM的mRNA，蛋白质和活性水平降低约40％。凝血酶抑制剂水rud素阻止了这些作用。这些结果表明，凝血级联反应的激活可能导致正反馈回路，该回路由凝血酶介导的对TM依赖性蛋白C激活的抑制。

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《Endothelium: Journal of endothelial cell research》 |2008年第3期|共6页
作者
Seguin C; Abid MR; Spokes KC; Aird WC;
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正文语种 eng
中图分类人体形态学;
关键词
Cells; Cultured; Endothelial Cells; Endothelium; Vascular; Gene Expression Regulation; 细胞; 培养的; 内皮; 血管; 基因表达调控; 蛋白酶抑制药; RNA; 信使; 凝血酶;

机译：Cells;Cultured;Endothelial Cells;Endothelium;Vascular;Gene Expression Regulation;细胞;培养的;内皮;血管;基因表达调控;蛋白酶抑制药;RNA;信使;凝血酶;

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专利

1. Thrombin downregulates thrombomodulin expression and activity in primary human endothelial cells. [J] . Seguin C, Abid MR, Spokes KC, Endothelium: Journal of endothelial cell research . 2008,第3期

机译：凝血酶下调人类原代内皮细胞中的血栓调节蛋白表达和活性。
2. Thrombomodulin modulates the mitogenic response to thrombin of human umbilical vein endothelial cells. [J] . Lafay M, Laguna R, Le-Bonniec BF, Thrombosis and Haemostasis: Journal of the International Society on Thrombosis and Haemostasis . 1998,第4期

机译：血栓调节蛋白调节人脐静脉内皮细胞对凝血酶的促有丝分裂反应。
3. Retinoic acid stimulates expression of thrombomodulin, a cell surface anticoagulant glycoprotein, on human endothelial cells. Differences between up-regulation of thrombomodulin by retinoic acid and cyclic AMP [J] . H Ishii, K Kizaki, M Kazama, The biochemical journal . 1992,第1期

机译：维甲酸可刺激血栓调节蛋白（一种细胞表面抗凝糖蛋白）在人内皮细胞上的表达。视黄酸和环状AMP上调血栓调节素的差异
4. Arterial shear stress downregulates thrombin receptor expression and attenuates thrombin-induced response in endothelial cells [C] . K. Nguyen, C. Patteson, M. Runge, IEEE Engineering in Medicine and Biology Annual Conference . 1999

机译：动脉剪切应力下调凝血酶受体表达并衰减内皮细胞中的凝血酶诱导的反应
5. The effect of laminar fluid flow on thrombomodulin activity and gene regulation in human endothelial cells. [D] . Panaro, Nicholas Joseph, III. 1993

机译：层流对人内皮细胞中血栓调节蛋白活性和基因调控的影响。
6. Retinoic acid stimulates expression of thrombomodulin a cell surface anticoagulant glycoprotein on human endothelial cells. Differences between up-regulation of thrombomodulin by retinoic acid and cyclic AMP. [O] . S Horie, K Kizaki, H Ishii, 1992

机译：维甲酸可刺激血栓调节蛋白（一种细胞表面抗凝糖蛋白）在人内皮细胞上的表达。视黄酸和环状AMP上调血栓调节素之间的差异。
7. Heterogeneous regulation of constitutive thrombomodulin or inducible tissue-factor activities on the surface of human saphenous-vein endothelial cells in culture following stimulation by interleukin-1, tumour necrosis factor, thrombin or phorbol ester. [O] . Archipoff, G, Beretz, A, Freyssinet, J M, 1991

机译：白细胞介素-1，肿瘤坏死因子，凝血酶或佛波酯刺激后，培养物中人隐静脉内皮细胞表面的血栓调节蛋白或可诱导的组织因子活性的异质调节。

Thrombin downregulates thrombomodulin expression and activity in primary human endothelial cells.

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