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Impedimetric immunosensor for detection of plum pox virus in plant extracts

机译：阻抗式免疫传感器检测植物提取物中的李子痘病毒

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This paper concerns the development of an immunosensor for detection of Plum pox virus in plant extracts. The sensor was based on gold electrodes modified with: 1,6-hexanedithiol, gold nanoparticles, anti-PPV IgG polyclonal antibody and BSA. It was used for determination of the virus in extracts from plum (Prunus domestica) and tobacco (Nicotiana benthamiana) leaves. The proposed electrochemical impedance spectroscopy (EIS) immunosensor displayed a very good detection limit of 10pg/mL and a wide dynamic range from 10pg PPV/mL to 200pg/mL. The presence of extracts from plant materials has no influence on the immunosensor's response. The immunosensor was capable of discriminating between samples from healthy plants and samples containing 0.01% of extract from infected plant material. The conditions for immunosensor regeneration are also presented.
机译：本文涉及用于检测植物提取物中梅花病毒的免疫传感器的开发。该传感器基于金电极，修饰后的电极为：1,6-己二硫醇，金纳米颗粒，抗PPV IgG多克隆抗体和BSA。它用于测定李子（李属）和烟草（本特烟草）叶提取物中的病毒。拟议的电化学阻抗谱（EIS）免疫传感器显示出非常好的10pg / mL的检测限和从10pg PPV / mL到200pg / mL的宽动态范围。来自植物材料的提取物的存在对免疫传感器的反应没有影响。免疫传感器能够区分健康植物样品和含有0.01％受感染植物材料提取物的样品。还介绍了免疫传感器再生的条件。

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