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首页> 外文期刊>Endocrine Research >Zonal differentiation in the rat adrenal cortex.
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Zonal differentiation in the rat adrenal cortex.

机译：大鼠肾上腺皮质区带分化。

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The factors that establish and maintain adrenocortical zonation are poorly understood. The capsular adrenal gland of the rat has been shown to develop into a functionally zoned tissue in autotransplanted glands in vivo. To examine this in vitro, capsular gland preparations (largely glomerulosa (zg) with some fasciculata (zf) were cultured in vitro in Eagles MEM (3.6mM K+) for 14 days. Zonal differentiation was determined by immunocytochemical localisation of inner zone antigen (IZA, zf/reticularis specific) and Pref-1 (zg specific). In the absence of further additions these preparations invariably maintained a good zonal arrangement of zg and zf over the whole period, though without significant cellular proliferation. Neither the daily addition of the stimulants, maximally 8.3mM potassium, 1nM ACTH, or 100nM angiotensin II (AII), or the AII type 1 receptor antagonist losartan (10microM) had any significant effect on the glands intrinsic capacity to maintain zonation in vitro. Aldosterone output declined rapidly under control conditions (3.6mM K+), but was stimulated by AII, or high K+ reaching a maximum after 7 days, and thereafter declined. However at higher K+ conditions (5.6mM) aldosterone was not supported by angiotensin II. Corticosterone secretion increased autonomously after 2 days in 3.6mM K+ then declined. At higher K+ conditions corticosterone rapidly declined. The factors studied had no effect on the inherent property of the adrenal gland to express the zg or zf phenotype. However the functional steroidogenic capacity of the adrenocortical cells was affected in a highly specific and complex manner by the added stimulants.
机译：建立和维持肾上腺皮质区带的因素了解甚少。已经显示，大鼠的荚膜肾上腺在体内会在自身移植的腺体内发育成功能分区的组织。为了进行体外检查，在Eagles MEM（3.6mM K +）中体外培养荚膜腺制剂（主要是肾小球（zg）和一些束膜（zf））14天，并通过内区抗原（IZA）的免疫细胞化学定位来确定区域分化。 ，zf / reticularis特异性）和Pref-1（zg特异性）在没有进一步添加的情况下，这些制剂在整个过程中始终保持zg和zf的良好区域分布，尽管没有明显的细胞增殖。兴奋剂，最大8.3mM钾，1nM ACTH或100nM血管紧张素II（AII）或AII 1型受体拮抗剂洛沙坦（10microM）对腺体在体外维持分区的内在能力有任何显着影响，醛固酮输出在控制下迅速下降条件下（3.6mM K +），但受到AII刺激，或高K +在7天后达到最大值，然后下降;但是在更高K +条件（5.6mM）下，醛固酮并没有由血管紧张素II支持。皮质类固醇分泌在3.6mM K +下2天后自主增加，然后下降。在较高的K +条件下，皮质酮迅速下降。研究的因素对表达zg或zf表型的肾上腺的固有特性没有影响。然而，肾上腺皮质细胞的功能性类固醇生成能力受到添加的刺激物的高度特异性和复杂性的影响。

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