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首页> 外文期刊>Biochimica et biophysica acta. Biomembranes >Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum
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Magnesium uptake of Arabidopsis transporters, AtMRS2-10 and AtMRS2-11, expressed in Escherichia coli mutants: Complementation and growth inhibition by aluminum

机译：大肠杆菌突变体中表达的拟南芥转运蛋白AtMRS2-10和AtMRS2-11的镁吸收：铝的互补和生长抑制

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摘要

Magnesium (Mg2+) plays a critical role in many physiological processes. Mg2+ transport systems in Salmonella have been well documented, but those in Escherichia coil have not been fully elucidated. We examined the effects of corA,mgtA, yhiD and corC gene deletion on Mg2+ transport in E. coli. We obtained every combination of double, triple and quadruple mutants. The corA and mgtA double mutant required addition of 10 mM Mg2+ to LuriaBertani (LB) medium for growth, and the corA, mgtA and yhiD triple mutant TM2 required a higher Mg2+ concentration. The Mg2+ requirement of the quadruple mutant was similar to that of TM2. The results demonstrated that either CorA or MgtA is necessary for normal E. coli growth in LB medium and that YhiD plays a role in Mg2+ transport under high Mg2+ growth conditions in E. coli. The Arabidopsis Mg2+ transporters, AtMRS2-10 and AtMRS2-11, were heterologously expressed in TM2 cells. TM2 cells expressing AtMRS2-10 and AtMRS2-11 could grow in LB medium that had been supplemented with 1 mM Mg2+ and without Mg2+ supplementation, respectively, and cell growth was inhibited by 2 mM AlCl3. The results indicated that the growth of TM2 expressing AtMRS2-10 and AtMRS2-11 reflected these AtMRS2 function for Mg2+ and aluminum. The E. coli TM2 cells are useful for functional analysis of Arabidopsis MRS2 proteins. (C) 2015 Elsevier B.V. All rights reserved.
机译：镁（Mg2 +）在许多生理过程中都起着至关重要的作用。沙门氏菌中的Mg2 +转运系统已有充分的文献记载，但埃希氏菌中的Mg2 +转运系统尚未完全阐明。我们检查了corA，mgtA，yhiD和corC基因缺失对大肠杆菌中Mg2 +转运的影响。我们获得了双重，三重和四重突变体的每种组合。 corA和mgtA双重突变体需要向LuriaBertani（LB）培养基中添加10 mM Mg2 +才能生长，而corA，mgtA和yhiD三重突变体TM2需要更高的Mg2 +浓度。四重突变体的Mg2 +需求量与TM2相似。结果表明，CorA或MgtA是LB培养基中正常大肠杆菌生长所必需的，并且YhiD在大肠杆菌中高Mg2 +生长条件下的Mg2 +转运中起作用。拟南芥Mg2 +转运蛋白AtMRS2-10和AtMRS2-11在TM2细胞中异源表达。表达AtMRS2-10和AtMRS2-11的TM2细胞可以在分别添加了1 mM Mg2 +和不添加Mg2 +的LB培养基中生长，并且细胞生长被2 mM AlCl3抑制。结果表明，表达AtMRS2-10和AtMRS2-11的TM2的生长反映了这些AtMRS2对Mg2 +和铝的功能。大肠杆菌TM2细胞可用于拟南芥MRS2蛋白的功能分析。 （C）2015 Elsevier B.V.保留所有权利。

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