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首页> 外文期刊>Environment and Ecology >Comparative Anaerobic Fermentation of Pure Glycerol and Crude Glycerol by Klebsiella spp. for the Production of 1,3-Propanediol
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Comparative Anaerobic Fermentation of Pure Glycerol and Crude Glycerol by Klebsiella spp. for the Production of 1,3-Propanediol

机译：Klebsiella spp比较纯甘油和粗甘油的厌氧发酵。用于生产1,3-丙二醇

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摘要

The Fed batch fermentation of 1, 3-propanediol (1. 3-PD) by Klebsiella spp. at different concentration of crude glycerol and pure glycerol, pH (6.5) and temperature (37"C) was investigated. The comparative production of 1,3-PDO was carried out from crude glycerol and pure glycerol at an initial concentration of 30 g/1 by isolated Klebsiella spp. strain. Before inoculation, At the initial cone, of extracted glycerol (30.49 g/1), No production of 1,3-PDO was observed while after inoculation. At the cone of 28.55g/1 of extracted glycerol, Very less production of 1,3 PDO (0.89 g/1) was recorded. After the incubatioi period of 2 h, at the maximum concentration df ex tracted glycerol (28.55 g/1). the minimum 1,3-PDO pro duction of 1.07 g/1 was recorded, while after47 h o incubation, at the minimum concentration of extractet glycerol (2.07 g/1), the maximum 1.3-PDO productior of 12.25 g/1 was observed. For the fermentation pro file of pure glycerol, Before inoculation, At the initia concentration of pure glycerol(30.12 g/1). Productior of 1,3-PDO of 1.12 g/1 was recorded while after inocu lation, At the concentration of 30.91 g/1 of pure glycerol, The less production of 1,3-PDO (1.29 g/1) was recorded. After the incubation period of 2 h, the maximum cone of pureglycerol (29.20) and minimum production of 1, 3-PDO (2.11 g/1) was observed, while after 48 h of incubation, at the concentration of 1.28 g/1 of pure glycerol, the maximum production of 1,1 PDO (10.54 g/1) was observed. As such as the incubation time was enhanced, the decreased in cone of glycerol and increased in production of 1,3-PDO was observed.
机译：克雷伯氏菌属的1，3-丙二醇（1.3-PD）的Fed分批发酵。研究了在不同浓度的粗甘油和纯甘油下的pH（6.5）和温度（37“ C）。由粗甘油和纯甘油在初始浓度为30 g / g的条件下进行了1,3-PDO的对比生产。 1分离的克雷伯菌属菌株，接种前，在提取锥的起始锥处（30.49 g / 1），接种后未观察到1,3-PDO的生成，在提取锥处28.55g / 1甘油，记录的1,3 PDO产量极少（0.89 g / 1），孵育2 h后，在最大浓度df提取的甘油（28.55 g / 1）下，最低1,3-PDO pro记录的转导量为1.07 g / 1，而在保温47小时后，在最低提取甘油浓度（2.07 g / 1）下，观察到最大1.3-PDO产量为12.25 g / 1。 ，接种前，在初始甘油浓度为30.12 g / 1时，可得到1.12 g / 1的1,3-PDO产物。接种后，在纯甘油浓度为30.91 g / 1时，记录的1,3-PDO产量较少（1.29 g / 1）。温育2小时后，观察到最大浓度的纯甘油（29.20）和最低产生1，3-PDO（2.11 g / 1），而温育48小时后，在1.28 g / 1的浓度下在纯甘油中，观察到最大产量为1,1 PDO（10.54 g / 1）。这样，如延长了孵育时间，观察到甘油的锥度降低和1，3-PDO产量增加。

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