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Entometabolomics: applications of modern analytical techniques to insect studies

机译：人体代谢物：现代分析技术在昆虫研究中的应用

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摘要

Metabolomic analyses can reveal associations between an organism's metabolome and further aspects of its phenotypic state, an attractive prospect for many life-sciences researchers. The metabolomic approach has been employed in some, but not many, insect study systems, starting in 1990 with the evaluation of the metabolic effects of parasitism on moth larvae. Metabolomics has now been applied to a variety of aspects of insect biology, including behaviour, infection, temperature stress responses, CO2 sedation, and bacteria-insect symbiosis. From a technical and reporting standpoint, these studies have adopted a range of approaches utilising established experimental methodologies. Here, we review current literature and evaluate the metabolomic approaches typically utilised by entomologists. We suggest that improvements can be made in several areas, including sampling procedures, the reduction in sampling and equipment variation, the use of sample extracts, statistical analyses, confirmation, and metabolite identification. Overall, it is clear that metabolomics can identify correlations between phenotypic states and underlying cellular metabolism that previous, more targeted, approaches are incapable of measuring. The unique combination of untargeted global analyses with high-resolution quantitative analyses results in a tool with great potential for future entomological investigations.
机译：代谢组学分析可以揭示生物体的代谢组与其表型状态的其他方面之间的关联，这对许多生命科学研究人员而言具有诱人的前景。从1990年开始，代谢组学方法已用于一些但不是很多昆虫研究系统中，从评估寄生虫对蛾幼虫的代谢影响开始。代谢组学现已应用于昆虫生物学的各个方面，包括行为，感染，温度应激反应，CO2镇静和细菌-昆虫共生。从技术和报告的角度来看，这些研究采用了一系列利用已建立的实验方法的方法。在这里，我们回顾当前的文献并评估昆虫学家通常使用的代谢组学方法。我们建议可以在几个方面进行改进，包括采样程序，减少采样和设备差异，使用样品提取物，统计分析，确认和代谢物鉴定。总体而言，很明显，代谢组学可以识别表型状态与潜在的细胞代谢之间的相关性，而以前的方法更具针对性，无法进行测量。非目标全局分析与高分辨率定量分析的独特结合，使得该工具具有巨大的潜力，可用于未来的昆虫学研究。

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