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首页> 外文期刊>Environmental and experimental botany >Arbuscular mycorrhizal fungi modulate the leaf transcriptome of a Populus alba L. clone grown on a zinc and copper-contaminated soil
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Arbuscular mycorrhizal fungi modulate the leaf transcriptome of a Populus alba L. clone grown on a zinc and copper-contaminated soil

机译：丛枝菌根真菌调节生长在锌和铜污染土壤上的白杨无性系的叶片转录组

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Significant improvement of growth associated with increased, rather than decreased, uptake of Cu and Zn has been observed in poplar plants inoculated with Glomus spp. as compared with non-mycorrhizal plants. The beneficial effect exerted by these arbuscular mycorrhizal fungi (AMF) is likely to be controlled by specific gene expression patterns in the plant. Until now, however, very little is known about the transcriptional changes which occur in response to heavy metals (HMs) in mycorrhizal vs. non-mycorrhizal poplar plants. In order to identify such HM- and/or AMF-induced changes in leaves of white poplar (Populus alba L.) plants grown, in the greenhouse, on Cu- and Zn-polluted soil, the cDNA-Amplified Fragment Length Polymorphism (AFLP) approach was adopted, resulting in the identification of a number of new differentially regulated genes. Transcript derived fragments (TDFs) mostly belonged to stress-related functional categories of defence and secondary metabolism. Genes belonging to different functional categories, plus other genes known to be related to HM stress (metallothioneins, phytochelatin synthase, glutathione synthase, arginine decarboxylase), were analysed by quantitative (q)RT-PCR. Transcript levels were generally down-regulated, or unaffected, in polluted soil compared with controls, the main exceptions being phytochelatin synthase and clathrin, and strongly up-regulated in the presence of AMF, especially Glomus mosseae. (C) 2011 Elsevier B.V. All rights reserved.
机译：在接种Glomus spp的杨树植物中，观察到与增加而不是减少吸收Cu和Zn有关的生长显着改善。与非菌根植物相比这些丛枝菌根真菌（AMF）发挥的有益作用很可能受植物中特定基因表达模式的控制。然而，到目前为止，对于在菌根和非菌根的杨树植物中对重金属（HMs）的响应而发生的转录变化知之甚少。为了鉴定这种HM和/或AMF诱导的在温室中的Cu和Zn污染土壤上生长的白杨（Populus alba L.）植物叶片中的这种变化，cDNA扩增的片段长度多态性（AFLP） ）方法被采用，从而鉴定出许多新的差异调节基因。转录本衍生片段（TDF）大部分属于与压力相关的防御和次级代谢功能类别。通过定量（q）RT-PCR分析了属于不同功能类别的基因，以及其他已知与HM应激相关的基因（金属硫蛋白，植物螯合素合酶，谷胱甘肽合酶，精氨酸脱羧酶）。与对照相比，污染土壤中的转录物水平通常被下调或不受影响，主要例外是植物螯合酶合酶和网格蛋白，并且在AMF（特别是Glomus mosseae）存在下强烈上调。 （C）2011 Elsevier B.V.保留所有权利。

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