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首页> 外文期刊>Environmental and experimental botany >Pb-induced ultrastructural alterations and subcellular localization of Pb in two species of Lespedeza by TEM-coupled electron energy loss spectroscopy
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Pb-induced ultrastructural alterations and subcellular localization of Pb in two species of Lespedeza by TEM-coupled electron energy loss spectroscopy

机译：TEM耦合电子能量损失谱法研究铅在两种Lespedeza中的超微结构变化和铅的亚细胞定位

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Transmission electron microscopy (TEM) coupled electron energy loss spectroscopy (EELS) was conducted in the present study, in order to explore mechanisms involved in Pb tolerance and detoxification in two species of Lespedeza (Fabaceae). Both Lespedeza chinensis and Lespedeza davidii have been recently shown to be suitable for chelate-assisted Pb phytoremediation. Ultrastructural alterations, Pb localization and speciation in leaf and root cells of both species were investigated after Pb exposure. Additionally, the influence of EDTA which facilitates Pb translocation in plants was investigated. In general, deleterious effects were more pronounced in root cells of both species than in leaf cells. Similar ultrastructural alterations such as vacuolization and dilation of the nuclear envelopes were induced in leaf and root cells of both species by Pb treatment. The effects were intensified with the addition of EDTA. Autophagy occurred only in leaf cells of L davidii after Pb and Pb plus EDTA treatment, and in root cells of both species after Pb treatment. Moreover, electron-dense deposits with variable shapes such as acicular, granulated, punctuated and amorphous, were present in cell walls, intercellular spaces, cytoplasm and vacuoles of root cells of both species after Pb and Pb plus EDTA treatment. These deposits contained Pb as identified via the Pb-M-4,M-5 edge by EELS in a 120 kV TEM. As phosphorus was always measured at the sites of Pb deposition, it is likely that Pb in Lespedeza is stored as lead phosphate particularly in cell walls and vacuoles. The occasional presence of S and Ca in Pb depositions may point to additional other Pb compounds. Our studies suggest that Pb is transported both in apoplastic and symplastic pathways, independent of the presence or absence of EDTA. Different possible detoxification strategies such as sequestration in cell walls or compartmentalization in vacuoles exist both in L chinensis and L. davidii. Only minor differences in ultrastructural alterations and Pb localization were observed between two species. (C) 2011 Elsevier B.V. All rights reserved.
机译：在本研究中进行了透射电子显微镜（TEM）耦合电子能量损失谱（EELS），目的是探讨莱斯佩德萨（Fabaceae）两种物种中Pb耐受性和排毒的机理。最近已证明中国小菜蛾和小菜蛾都适用于螯合物辅助的铅植物修复。暴露于铅后，研究了两种物种的叶和根细胞中的超微结构变化，铅的定位和形态。此外，研究了EDTA促进植物中Pb易位的影响。通常，在两种物种的根细胞中，有害作用比叶细胞中更为明显。通过铅处理，在两种物种的叶和根细胞中诱导了类似的超微结构改变，例如空泡化和核被膜的扩张。加入EDTA可增强效果。自噬仅发生在铅和铅加EDTA处理后的大卫氏L的叶子细胞中，以及两种物质在铅处理后的根细胞中。此外，Pb和Pb加上EDTA处理后，这两种物种的根细胞的细胞壁，细胞间隙，细胞质和液泡中都存在形状多样的电子致密沉积物，如针状，颗粒状，点状和无定形。这些沉积物包含Pb，通过EELS在120 kV TEM中通过Pb-M-4，M-5边缘鉴定。由于总是在Pb的沉积位置测量磷，因此Lespedeza中的Pb可能以磷酸铅的形式存储，尤其是在细胞壁和液泡中。 Pb沉积物中偶尔存在S和Ca可能表明存在其他Pb化合物。我们的研究表明，Pb既可以通过质外途径又可以通过共生途径进行运输，而与是否存在EDTA无关。在中华绒螯蟹和大卫乳杆菌中都存在不同的可能的排毒策略，例如在细胞壁中隔离或在液泡中分隔。在两个物种之间，仅观察到超微结构改变和Pb定位的微小差异。 （C）2011 Elsevier B.V.保留所有权利。

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