This study tested our hypothesis that during an 80-km Research Ride in 2002 R2 horses that did not finish NF the ride would have elevated muscle enzyme activities in the blood and changes in biomarkers of oxidative stress as compared to horses that finished F the ride. These results were then compared to previous rides - Old Dominion OD and the Research Ride 2001 Rl. For R2, 40 mostly Arabian horses competed and had blood samples collected before, at 27, 48 and 80km, and 170 to 190min afterthe 80-km race. Blood was collected similarly in Rl and OD. Blood was analysed for plasma lipid hydroperoxides LPO, a-tocopherol TOC, creatine kinase CK, aspartate aminotransferase AST, red and white blood cell total glutathione GSH-T and glutathione peroxidase GPx. Data were analysed using a repeated measure ANOVA in SAS. Associations between muscle enzymes and antioxidant status were determined using Pearson's or Spearman's correlations. Activities of CK and AST were higher P < 0.05 before, during and after the ride in NF than in F; however, TOC, LPO, GSH-T and GPx were not different. In R2, negative correlations were found with GPx and CK r = — 0.21; P= 0.005, GPx and AST r = -0.15; P = 0.05, and a positive correlation was foundwith GSH-T and CK r = 0.18; P = 0.02. Values of CK, LPO, GPx and GSH-T were higher P < 0.05 in R2 than in Rl or OD. The overall comparison of 80-km endurance races suggests the importance of considering the horse's fitness, terrain, ambient conditions and calibre of race when interpreting results from markers of oxidative stress and muscle enzyme leakage.
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机译：这项研究检验了我们的假设，即与2002年完成F赛的马匹相比，在2002年R2骑行中未完成NF的马在血液中的肌肉酶活性升高，并且氧化应激的生物标志物发生了变化。然后将这些结果与以前的游乐设施-Old Dominion OD和Research Ride 2001 R1进行比较。对于R2，40匹主要是阿拉伯马匹参加比赛，并在80公里比赛之后的27、48和80公里以及170至190分钟之前收集了血液样本。在R1和OD中类似地收集血液。分析血液中的血浆脂质氢过氧化物LPO，α-生育酚TOC，肌酸激酶CK，天冬氨酸转氨酶AST，红细胞和白细胞总谷胱甘肽GSH-T和谷胱甘肽过氧化物酶GPx。使用SAS中的重复测量ANOVA分析数据。肌肉酶和抗氧化剂状态之间的关联是使用Pearson或Spearman相关性确定的。 NF骑行前后，CK活动和AST活动均高于F; P <0.05。但是，TOC，LPO，GSH-T和GPx并无不同。在R2中，发现GPx和CK r =-0.21呈负相关; P = 0.005，GPx和AST r = -0.15; P = 0.05，与GSH-T和CK r = 0.18呈正相关; P ＝ 0.02。 R2中的CK，LPO，GPx和GSH-T的值高于R1或OD中的P ＜0.05。对80公里耐力赛的整体比较表明，在解释氧化应激和肌肉酶渗漏标记的结果时，考虑马匹的体能，地形，环境条件和赛程的重要性非常重要。
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