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Isolation of cDNA encoding a novel human CC chemokine NCC-4 /LEC

机译:编码新型人CC趋化因子NCC-4 / LEC的cDNA的分离

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We have determined the entire sequence of human cDNA encoding a novel CC chemokine NCC-4 by 5' and 3' RACE methods. Two types of transcripts, 579 bp and 1503 bp long, respectively, are generated through alternative polyadenylation sites. Both species contain an open reading frame encoding 120 amino acids with 19-38% identity to other human CC chemokines. The short and long transcripts are expressed highly selectively in the liver at nearly equivalent levels. There seems to be one copy of the gene per haploid genome. We now designate NCC-4 as LEC from liver-expressed chemokine.
机译:我们已经通过5'和3'RACE方法确定了编码新型CC趋化因子NCC-4的人cDNA的完整序列。通过替代的聚腺苷酸化位点分别产生两种类型的转录本,分别长579 bp和1503 bp。两种物种都包含一个开放阅读框,该框编码120个氨基酸,与其他人CC趋化因子具有19-38%的一致性。短和长转录本在肝脏中以几乎相等的水平高度选择性表达。每个单倍体基因组似乎都有一个基因拷贝。现在,我们从肝脏表达的趋化因子中将NCC-4指定为LEC。

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